NDUFS1 (NM_005006) Human 3' UTR Clone

CAT#: SC212473

3`UTR clone of NADH dehydrogenase (ubiquinone) Fe-S protein 1 75kDa (NADH-coenzyme Q reductase) (NDUFS1) nuclear gene encoding mitochondrial protein for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NDUFS1
• Synonyms: CI-75k; CI-75Kd; MC1DN5; PRO1304
• ACCN: NM_005006
• Insert Size: 1108 bp
• Sequence Data:
  Insert Sequence

  >SC212473 3'UTR clone of NM_005006
  The sequence shown below is from the reference sequence of NM_005006. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGCAGTAGAGGAACCATCCATATGCTGAAGCTTCTACTAGGATCCCAGTTTTGCCGCAGATAATTAATG
  GACAACTGTAGTGCAGTGATCCTTTACAGGTTTATTTCTTTGTAAAAAAAAATAATAATAATTTGAATCA
  TGTAATATTTAAGGTTATACTATGCCTATTTGAAAATGATATTAGTTATCAACTTTGCAGTTTGAAAAAC
  ATGTATTGTGTGTAAAGGTTAAATAACAAAACTATGCAGATGCTCTTAAAAGCATTGATAACCTTTGTGA
  CGAACATAAAGAGATCCTTAAATTATGAGTTGTTGGCTTATCTTCATAAATAATTTGTCTGTAAAATGGA
  TGAAATGAAAAGAGGTTCAATTAAAACCTACTTTTTTCTAGTGCTAAAGAAAAGATTTAAGCACCTTGTC
  AAGCTGGGTAAATAGGAAAAATACATAATCATGCTCAGATATGTATCTAGGATAATTATAATTAATAATA
  ATCATAGTAACAATGGCTAATGATAATTTAGCTTTATTATATGTGCTAAGCACTCTGGTTTTACATGCAT
  TATCTTCTTTTGTTCTTGCTACAACCCTGTGAGATAGTAGTATTATCTCATTTTACTGATGAAGACTGAA
  GCCTAGGTATATTAAATAGCTTGCCCAAGGCCACACAGCAAAAGTCACCAACTCAAACCTACTTCTTATT
  TACTCCAAAGCCTGTTGTTCTTAACTGCAACATATTATTTCGTCTCATTAATGTTGATTCTATAGGTTGT
  TACTTCTAAAAATTAGTATTGAGTTTAATGGTGAACACATTTTTCTATTTTCTCTTGAATCTGCTTCTAT
  AATGTCATGGTGATTTATGTGGCTTTTTTTTTTTTATAAGTTATACATGTATGCATGTATACTTATGAGA
  CCTCCCTTGGAATGAGGGAGGTCTCAAGAGATATAATTTAGATTCTCATTGATGTTCTGTATTCATTATC
  CTAACACCATCTGTAGTGTTAAATCAACTAAATTATTTCAGCAATAGGAGACAAAACAACCAGCTTTCAT
  AATTTTTAATTGTCAAAACCAAAAGGAATCAGAATAAGGATCACTGAGAATTTAAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005006.5
• Summary: 'The protein encoded by this gene belongs to the complex I 75 kDa subunit family. Mammalian complex I is composed of 45 different subunits. It locates at the mitochondrial inner membrane. This protein has NADH dehydrogenase activity and oxidoreductase activity. It transfers electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone. This protein is the largest subunit of complex I and it is a component of the iron-sulfur (IP) fragment of the enzyme. It may form part of the active site crevice where NADH is oxidized. Mutations in this gene are associated with complex I deficiency. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2011]'
• Locus ID: 4719