PPP2R5A (NM_006243) Human 3' UTR Clone

CAT#: SC212496

3`UTR clone of protein phosphatase 2 regulatory subunit B' alpha isoform (PPP2R5A) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PPP2R5A
• Synonyms: B56A; B56alpha; PR61A
• ACCN: NM_006243
• Insert Size: 1087 bp
• Sequence Data:
  Insert Sequence

  >SC212496 3'UTR clone of NM_006243
  The sequence shown below is from the reference sequence of NM_006243. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTCAGCAATACAAGTGCCGAATAAAAAAAAAGCCTCCCACCTCTGCCGGATAGGCAGAGTTTTGTATGCT
  TTTTTGAAATATGTAAAAATTACAAAACAAACCTCATCAGTATAATATAATTAAAAGGCCAATTTTTTCT
  GGCAACTGTAAATGGAAAAATATATGGACTAAACGTAGCCCTGTGCTGTATCATGGCCATAGTATATTGT
  AACCTTTGTCTAATCATTGGATTTATTGTGTCACTTCTGAAGTTTCACAGAAATGAATGAATTTTATCAT
  CTATGATATGAGTGAGATAATTATGGGAGTGGTAAGAATTATGACTTGAATTCTTCTTTGATTGTGTTGC
  ACATAGATATGGTAGTCTGCTCTGTATATTTTTCCCTTTTATAATGTGCTTTTCACACTGCTGCAAACCT
  TAGTTACATCCTAGGAAAAAATACTTCCTAAAATAAAACTAAGGTATCATCCTTACCCTTCTCTTTGTCT
  CACCCAGAAATATGATGGGGGGAATTACCTGCCCTAACCCCTCCCTCAATAAATACATTACTGTACTCTG
  GAATTTAGGCAAAACCTTAAATCTCCAGGCTTTTTAAAGCACAAAATATAAATAAAAGCTGGGAAAGTAA
  ACCAAAATTCTTCAGATTGTTCCTCATGAATATCCCCCTTCCTCTGCAATTCTCCAGAGTGGTAACAGAT
  GGGTAGAGGCAGCTCAGGTGAATTACCCAGCTTGCCTCTCAATTCATTCCTCCTCTTCCTCTCAAAGGCT
  GAAGGCAGGGCCTTTCCAGTCCTCACAACCTGTCCTTCACCTAGTCCCTCCTGACCCAGGGATGGAGGCT
  TTGAGTCCCACAGTGTGGTGATACAGAGCACTAGTTGTCACTGCCTGGCTTTATTTAAAGGAACTGCAGT
  AGGCTTCCTCTGTAGAGCTCTGAAAAGGTTGACTATATAGAGGTCTTGTATGTTTTTACTTGGTCAAGTA
  TTTCTCACATCTTTTGTTATCAGAGTACCATTCCAATCTCTTAACTTGCAGTTGTGTGGAAAACTGTTTT
  GTAATGAAAGATCTTCATTGGGGGATTGAGCAGCATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006243.2
• Summary: 'The product of this gene belongs to the phosphatase 2A regulatory subunit B family. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This gene encodes an alpha isoform of the regulatory subunit B56 subfamily. Alternative transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Dec 2010]'
• Locus ID: 5525