ASAH1 (NM_177924) Human 3' UTR Clone

CAT#: SC212562

3`UTR clone of N-acylsphingosine amidohydrolase (acid ceramidase) 1 (ASAH1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ASAH1
• Synonyms: AC; ACDase; ASAH; PHP; PHP32; SMAPME
• ACCN: NM_177924
• Insert Size: 1117 bp
• Sequence Data:
  Insert Sequence

  >SC212562 3'UTR clone of NM_177924
  The sequence shown below is from the reference sequence of NM_177924. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACCCTTGTATAGGTTGGTGAGCACACGTCTGGCCTACAGAATGCGGCCTCTGAGACATGAAGACACCAT
  CTCCATGTGACCGAACACTGCAGCTGTCTGACCTTCCAAAGACTAAGACTCGCGGCAGGTTCTCTTTGAG
  TCAATAGCTTGTCTTCGTCCATCTGTTGACAAATGACAGATCTTTTTTTTTTCCCCCTATCAGTTGATTT
  TTCTTATTTACAGATAACTTCTTTAGGGGAAGTAAAACAGTCATCTAGAATTCACTGAGTTTTGTTTCAC
  TTTGACATTTGGGGATCTGGTGGGCAGTCGAACCATGGTGAACTCCACCTCCGTGGAATAAATGGAGATT
  CAGCGTGGGTGTTGAATCCAGCACGTCTGTGTGAGTAACGGGACAGTAAACACTCCACATTCTTCAGTTT
  TTCACTTCTACCTACATATTTGTATGTTTTTCTGTATAACAGCCTTTTCCTTCTGGTTCTAACTGCTGTT
  AAAATTAATATATCATTATCTTTGCTGTTATTGACAGCGATATAATTTTATTACATATGATTAGAGGGAT
  GAGACAGACATTCACCTGTATATTTCTTTTAATGGGCACAAAATGGGCCCTTGCCTCTAAATAGCACTTT
  TTGGGGTTCAAGAAGTAATCAGTATGCAAAGCAATCTTTTATACAATAATTGAAGTGTTCCCTTTTTCAT
  AATTACTCTACTTCCCAGTAACCCTAAGGAAGTTGCTAACTTAAAAAACTGCATCCCACGTTCTGTTAAT
  TTAGTAAATAAACAAGTCAAAGACTTGTGGAAAATAGGAAGTGAACCCATATTTTAAATTCTCATAAGTA
  GCATTCATGTAATAAACAGGTTTTTAGTTTGTTCTTCAGATTGATAGGGAGTTTTAAAGAAATTTTAGTA
  GTTACTAAAATTATGTTACTGTATTTTTCAGAAATCAAACTGCTTATGAAAAGTACTAATAGAACTTGTT
  AACCTTTCTAACCTTCACGATTAACTGTGAAATGTACGTCATTTGTGCAAGACCGTTTGTCCACTTCATT
  TTGTATAATCACAGTTGTGTTCCTGACACTCAATAAACAGTCACTGGAAAGAGTGCCAGTCAGCAGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_177924.3
• Summary: 'This gene encodes a member of the acid ceramidase family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. Processing of this preproprotein generates alpha and beta subunits that heterodimerize to form the mature lysosomal enzyme, which catalyzes the degradation of ceramide into sphingosine and free fatty acid. This enzyme is overexpressed in multiple human cancers and may play a role in cancer progression. Mutations in this gene are associated with the lysosomal storage disorder, Farber lipogranulomatosis, and a neuromuscular disorder, spinal muscular atrophy with progressive myoclonic epilepsy. [provided by RefSeq, Oct 2015]'
• Locus ID: 427