INPP5E (NM_019892) Human 3' UTR Clone

CAT#: SC212571

3`UTR clone of inositol polyphosphate-5-phosphatase 72 kDa (INPP5E) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: INPP5E
• Synonyms: CORS1; CPD4; JBTS1; MORMS; pharbin; PPI5PIV
• ACCN: NM_019892
• Insert Size: 1087
• Sequence Data:
  Insert Sequence

  >SC212571 3'UTR clone of NM_019892
  The sequence shown below is from the reference sequence of NM_019892. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTCAGAACTCCAGCACCATCTGCTCCGTTTCTTGAAGTTTGCTGAACGAGGACTCACAGCTGCAACGTGG
  GGTGATTGTATTGATCAAAACCCACTGGGAAGGACAAAGAGTTTGCCGCCTTTCGGGGATCCGAGGGACT
  GTGGCGACCGTGCCTCTGTGCCAGCGTCCCAGGAAGGAAGCCAACCCTGAGCGAGCCTGTCCTCTGTGGC
  AGGTCCACACGGTGTGGGTGGGCAGGGCTTGGACCCCCGTCTCCATGGCAGGTCCATACAGCATGGGTGG
  CAGGGTTTGGACCCGCCCAGCAGCACCACGGACCCCAGCCACTCTCGGGGGCAGACGTCAGAATCCGTTC
  CTGAGCAGCTCCTGTGCCCTGGGGGCAGTCACAGAGCCCCCCAACACCCCCGTGCTCTGCACCAGCCTCT
  CCCTCCACACCCGAAGCAGGCGTCCATCTGTGTCCTCCTGGCAGCCCCTCAAACACACACCACCCCATGT
  CACACGGGTATCCAGAGCAAGGCTGGATCTGGGCTAGGTGACCCCTGGGGCCTCAGACCTCTGAGTGGGC
  CCCAGAGCCCAGCTGAGGAAGCCACACAGCCTCCAGGACGTGGCACGGTTCCCCTCCACTTGGCTGCAAG
  TCGCCTGCCATGGGGCGAGGAGGCCTGCACCCCAGTATAAGGGCCTCCCCAGCTGAGTAAGAAGCTGCTT
  CCCCTCCTCTCATAGGCCAAGCCTATTGTGTGAAACCATCTCATGGTCTTGGTGACGTAGACCATTTTTG
  AAACCGTCTCATGGTCTTGGTGACGTAGACCGTTTGCTTCTTTAACTCCAGCCGCGGAATGACATTAGTG
  GAACCGGGCTAGGGAACTGCTGGAAGTTCAGGATGCCACCACCTTGAACACCTAGGCCAGGGATCCCCAC
  CATGTCCCGGGTTTCTTTCTTCGAGAGTATAGAACCGTTCATTCTTGCTTTGTGTCCCATTCCATCTCTT
  GAAAAAATGTAGTCTTTGAATGTGTGAAAATCTAGGGACATTCAATCTAGTCTTTTAAATTATTTTTCCA
  TATTTATATTTTTAAATAAATGCATATATTGAGTCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_019892.3
• Summary: The protein encoded by this gene is an inositol 1,4,5-trisphosphate (InsP3) 5-phosphatase. InsP3 5-phosphatases hydrolyze Ins(1,4,5)P3, which mobilizes intracellular calcium and acts as a second messenger mediating cell responses to various stimulation. Studies of the mouse counterpart suggest that this protein may hydrolyze phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,5-bisphosphate on the cytoplasmic Golgi membrane and thereby regulate Golgi-vesicular trafficking. Mutations in this gene cause Joubert syndrome; a clinically and genetically heterogenous group of disorders characterized by midbrain-hindbrain malformation and various associated ciliopathies that include retinal dystrophy, nephronophthisis, liver fibrosis and polydactyly. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]
• Locus ID: 56623