EP300 (NM_001429) Human 3' UTR Clone

CAT#: SC212583

3`UTR clone of E1A binding protein p300 (EP300) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EP300
• Synonyms: KAT3B; MKHK2; p300; RSTS2
• ACCN: NM_001429
• Insert Size: 1151 bp
• Sequence Data:
  Insert Sequence

  >SC212583 3'UTR clone of NM_001429
  The sequence shown below is from the reference sequence of NM_001429. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCTCACAGAGTACACTAGACATACACTAGAGACACCTTGTAGTATTTTGGGAGCAAAAAAATTATTTT
  CTCTTAACAAGACTTTTTGTACTGAAAACAATTTTTTTGAATCTTTCGTAGCCTAAAAGACAATTTTCCT
  TGGAACACATAAGAACTGTGCAGTAGCCGTTTGTGGTTTAAAGCAAACATGCAAGATGAACCTGAGGGAT
  GATAGAATACAAAGAATATATTTTTGTTATGGCTGGTTACCACCAGCCTTTCTTCCCCTTTGTGTGTGTG
  GTTCAAGTGTGCACTGGGAGGAGGCTGAGGCCTGTGAAGCCAAACAATATGCTCCTGCCTTGCACCTCCA
  ATAGGTTTTATTATTTTTTTTAAATTAATGAACATATGTAATATTAATAGTTATTATTTACTGGTGCAGA
  TGGTTGACATTTTTCCCTATTTTCCTCACTTTATGGAAGAGTTAAAACATTTCTAAACCAGAGGACAAAA
  GGGGTTAATGTTACTTTAAAATTACATTCTATATATATATAAATATATATAAATATATATTAAAATACCA
  GTTTTTTTTCTCTGGGTGCAAAGATGTTCATTCTTTTAAAAAATGTTTAAAAAAAAAAAAAAACTGCCTT
  TCTTCCCCTCAAGTCAACTTTTGTGCTCCAGAAAATTTTCTATTCTGTAAGTCTGAGCGTAAAACTTCAA
  GTATTAAAATAATTTGTACATGTAGAGAGAAAAATGACTTTTTCAAAAATATACAGGGGCAGCTGCCAAA
  TTGATGTATTATATATTGTGGTTTCTGTTTCTTGAAAGAATTTTTTTCGTTATTTTTACATCTAACAAAG
  TAAAAAAATTAAAAAGAGGGTAAGAAACGATTCCGGTGGGATGATTTTAACATGCAAAATGTCCCTGGGG
  GTTTCTTCTTTGCTTGCTTTCTTCCTCCTTACCCTACCCCCCACTCACACACACACACACACACACACAC
  ACACACACACACACACACTTTCTATAAAACTTGAAAATAGCAAAAACCCTCAACTGTTGTAAATCATGCA
  ATTAAAGTTGATTACTTATAAATATGAACTTTGGATCACTGTATAGACTGTTAAATTTGATTTCTTATTA
  CCTATTGTTAAATAAACTGTGTGAGACAGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001429.3
• Summary: 'This gene encodes the adenovirus E1A-associated cellular p300 transcriptional co-activator protein. It functions as histone acetyltransferase that regulates transcription via chromatin remodeling and is important in the processes of cell proliferation and differentiation. It mediates cAMP-gene regulation by binding specifically to phosphorylated CREB protein. This gene has also been identified as a co-activator of HIF1A (hypoxia-inducible factor 1 alpha), and thus plays a role in the stimulation of hypoxia-induced genes such as VEGF. Defects in this gene are a cause of Rubinstein-Taybi syndrome and may also play a role in epithelial cancer. [provided by RefSeq, Jul 2008]'
• Locus ID: 2033