Amphiphysin (AMPH) (NM_139316) Human 3' UTR Clone

CAT#: SC212664

3`UTR clone of amphiphysin (AMPH) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AMPH
• Synonyms: AMPH1
• ACCN: NM_139316
• Insert Size: 1137 bp
• Sequence Data:
  Insert Sequence

  >SC212664 3'UTR clone of NM_139316
  The sequence shown below is from the reference sequence of NM_139316. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCCAGAGAACTTCACCCGACGCTTAGATTAGGGCAACAAGTACTGCAAGAAGGAGCTCAGTTACGGGGTT
  TTTAAACCTTCATGAAAACCTGAAGAGTTCACTTTTGTTATTATGCTCTTAATGATTTACAGACTGATGC
  CAGACAAACCTTGGGAAGATGTATCAATGGAGCATGTGTGCAAAAAAATGTAAGAGGAAAAAAAGTAAAT
  TAAGGAAAAAATCCTCACTCATTCCCATGTTGTCAATAACAGGTTTGTTTGTGCTTTGTCCAAGCTGTGG
  AGACTCTTGTACTTGATCCCCTCCCACCAATTCTAAAGTAGCTTTCTCCAGATCAGACTTTAATTTCTCT
  GCATCAGGTGTATCGTATTGAGTGGCTGCCCTGCAGAAGATGTGATGAATTATCCTGTAGTACAAGATTA
  TCTTATTCCCCTGCCCAGGTGTGAGCACGTGCTCTCGCTCCCTTTCAGGTTTACTATGTTTAAAACTAAA
  CTGCTGCAAAAGTTCTCAATGTTCTTTTCCAACACATCTATATATATATGGACACACAGTCACGCAAGCT
  AGTTCCCATGACTCCTGGATCTATGTGTATGCAGAAGGCTACATATACATAGTTGCTTCTTTCCTTTCCA
  TTGTAGCTCTTTGCCTCTCAATTGTCATTGATACATGCATTGCTCTTATTCTGTCTGGCATTACAATTGC
  AGTTTTGCCTAATGGCAAAACCAGCCATCCCATTGGCAAAAGAGTCAACAGTATCTGTGTTTGTACTGTG
  CAGTTTTCAGGATGAGATGACAGATAGCACGACTACACTGAGGCACCCTCAGAGAAGGCAGGGCCTGATT
  TATCTAGCTGTCCAGCCGCTGTGGCTGGAGGTAGGCTAGTTGGGATGGGCAGCTAAGCAGTCTTCTCTTC
  TGCCTTTATGAGATTGGGTAGAAAGATGGGGCATATTCATCTACCCCCAAAAGAACACTATAAAAACAGC
  TCCTCTATATTTCCACCTCTTTCTATTTATGCAACTCATCTTCACAGATTGTTCTAAAGTAAAATAGATG
  GTGTATGCTGTATCCACAAATCATCTGTAACAATTATGTTTTCAGTGCTGTGTCATTCCAAATAAACCTT
  TGGTAATCTTCAACGAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_139316.1
• Summary: 'This gene encodes a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11.[provided by RefSeq, Nov 2010]'
• Locus ID: 273