LEF1 (NM_001130714) Human 3' UTR Clone

CAT#: SC212757

3`UTR clone of lymphoid enhancer-binding factor 1 (LEF1) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LEF1
• Synonyms: LEF-1; TCF1ALPHA; TCF7L3; TCF10
• ACCN: NM_001130714
• Insert Size: 1140
• Sequence Data:
  Insert Sequence

  >SC212757 3'UTR clone of NM_001130714
  The sequence shown below is from the reference sequence of NM_001130714. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGACCTCTTCTGGAGATGGAAGCTTGTTGAAAACCCAGACTGTCTCCACGGCCTGCCCAGTCGACCCCA
  AAGGAACACTGACATCAATTTTACCCTGAGGTCACTGCTAGAGACGCTGATCCATAAAGACAATCACTGC
  CAACCCCTCTTTCGTCTACTGCAAGAGCCAAGTTCCAAAATAAAGCATAAAAAGGTTTTTTAAAAGGAAA
  TGTAAAAGCACATGAGAATGCTAGCAGGCTGTGGGGCAGCTGAGCAGCTTTTCTCCCCTCATATCTGCGT
  GCACTTCCCAGAGCATCTTGCATCCAAACCTGTAACCTTTCGGCAAGGACGGTAACTTGGCTGCATTTGC
  CTGTCATGCGCAACTGGAGCCAGCAACCAGCACATCCATCAGCACCCCAGTGGAGGAGTTCATGGAAGAG
  TTCCCTCTTTGTTTCTGCTTCATTTTTCTTTCTTTTCTTTTCTCCTAAAGCTTTTATTTAACAGTGCAAA
  AGGATCGTTTTTTTTTGCTTTTTTAAACTTGAATTTTTTTAATTTACACTTTTTAGTTTTAATTTTCTTG
  TATATTTTGCTAGCTATGAGCTTTTAAATAAAATTGAAAGTTCTGGAAAAGTTTGAAATAATGACATAAA
  AAGAAGCCTTCTTTTTCTGAGACAGCTTGTCTGGTAAGTGGCTTCTCTGTGAATTGCCTGTAACACATAG
  TGGCTTCTCCGCCCTTGTAAGGTGTTCAGTAGAGCTAAATAAATGTAATAGCCAAACCCACTCTGTTGGT
  AGCAATTGGCAGCCCTATTTCAGTTTATTTTTTCTTCTGTTTTCTTCTTTTCTTTTTTTAAACAGTAAAC
  CTTAACAGATGCGTTCAGCAGACTGGTTTGCAGTGAATTTTCATTTCTTTCCTTATCACCCCCTTGTTGT
  AAAAAGCCCAGCACTTGAATTGTTATTACTTTAAATGTTCTGTATTTGTATCTGTTTTTATTAGCCAATT
  AGTGGGATTTTATGCCAGTTGTTAAAATGAGCATTGATGTACCCATTTTTTAAAAAAGCAAGGCACAGCC
  TTTGCCCAAAACTGTCATCCTAACGTTTGTCATTCCAGTTTGAGTTAATGTGCTGAGCATTTTTTTAAAA
  GAAGCTTTGTAATAAAACAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001130714.2
• Summary: This gene encodes a transcription factor belonging to a family of proteins that share homology with the high mobility group protein-1. The protein encoded by this gene can bind to a functionally important site in the T-cell receptor-alpha enhancer, thereby conferring maximal enhancer activity. This transcription factor is involved in the Wnt signaling pathway, and it may function in hair cell differentiation and follicle morphogenesis. Mutations in this gene have been found in somatic sebaceous tumors. This gene has also been linked to other cancers, including androgen-independent prostate cancer. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]
• Locus ID: 51176