ORC2 (NM_006190) Human 3' UTR Clone

CAT#: SC212767

3`UTR clone of origin recognition complex subunit 2-like (yeast) (ORC2L) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ORC2
• Synonyms: ORC2L
• ACCN: NM_006190
• Insert Size: 1129 bp
• Sequence Data:
  Insert Sequence

  >SC212767 3'UTR clone of NM_006190
  The sequence shown below is from the reference sequence of NM_006190. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAAGAAGAGGAGGCTTGAAGCTTTCCTTTATTCTTGAATCTCCCATGGAAGGGTTGTACCCCAGCTGCC
  ACTCCTCTAGTTGAAAGTGTTGTGTTTACATCTGACATTAAATTATTTTTCCAGCATACAAGATTTAAAT
  TTGGGAAGGGGGGGATGTCCTCAATTAGAACTTTTTGATCAGCCTGGCTGGTACCGTCTAGTACTATGCA
  GCGGTCCTCAAGTTGGAGAAAATGTGCCTTTCATTCATTACCTCTCTGGAGACTTCTTGCTGGAATGAAC
  AGTGTGCTCAGGGACTATTTGGAACTGGATGTTTTTGAATTATTTTATACTTAGAGATATTCTGAATTTT
  TTGAGGGCCTTTTAACACTCCCCGAGCTGATTGTTTGCAAGTGTGTTTGTTCCAGAGTGTGGAAGTATAA
  AGACATGGGCATCACGTAAATTGGTTTTGTTTGCTATTCTGTGTGTCAGAACCAACGAGTGTAATGGAGA
  GGGCAGGTCATCTCTTATTGTTTCTAAAACAACTTAAAAGGTGTAGATTGGGAAGAGGTGAGTGATCCAG
  CTTTCTCCTTTTGGATTGAGGCTATGTACTTGGTGGGGGCAGGGGAGGGAATATATTATAATACTATTCA
  GTTGGGATAATGGGAAAAACAGAGTATATAGGGTATCTACCCAGCCTAGAAAGCACAGGAACAATACGTC
  ATATATTTGGAACAGTTATTGTCTGTGCCATGACCTTCATGATACCAGTGAGAAGCCAGGCTAGAGAAAT
  AAAATCCTGAATTACATTTTAGTAATTGTTTTCAAGACAACAAAAAATAAAACATTTCATTATTCTTGTA
  TATTGACATATTTTTCTTTTCTTGAACAACATGCAGGTGAGTAGCATATTAATGGCTGGCTGTAAATTAA
  TTTTGATTCCTCTGTCAAGTAGACCTAAAACTCACTGTAAGATATTTCAGTTTTTTCCTTAAAGTGATGA
  TTTCAAACCTCCTGAATATTTGCGCGAGACTTTTTTCTTAGGAAAGAAAAAAGGCTCACCTTGGCTACTG
  TTGTCAGCTTACAGTCATCACATACCCCCTGAAATATTTGACACTTGGAAATGAAGTTCTTCCTGAAAAT
  ATCACTGCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_006190.3
• Summary: 'The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. This protein forms a core complex with ORC3, -4, and -5. It also interacts with CDC45 and MCM10, which are proteins known to be important for the initiation of DNA replication. This protein has been demonstrated to specifically associate with the origin of replication of Epstein-Barr virus in human cells, and is thought to be required for DNA replication from viral origin of replication. Alternatively spliced transcript variants have been found, one of which is a nonsense-mediated mRNA decay candidate. [provided by RefSeq, Oct 2010]'
• Locus ID: 4999