Cytochrome P450 3A4 (CYP3A4) (NM_017460) Human 3' UTR Clone

CAT#: SC212797

3`UTR clone of cytochrome P450 family 3 subfamily A polypeptide 4 (CYP3A4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP3A4
• Synonyms: CP33; CP34; CYP3A; CYP3A3; CYPIIIA3; CYPIIIA4; HLP; NF-25; P450C3; P450PCN1
• ACCN: NM_017460
• Insert Size: 1141 bp
• Sequence Data:
  Insert Sequence

  >SC212797 3'UTR clone of NM_017460
  The sequence shown below is from the reference sequence of NM_017460. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACCGTAAGTGGAGCCTGAATTTTCCTAAGGACTTCTGCTTTGCTCTTCAAGAAATCTGTGCCTGAGAACA
  CCAGAGACCTCAAATTACTTTGTGAATAGAACTCTGAAATGAAGATGGGCTTCATCCAATGGACTGCATA
  AATAACCGGGGATTCTGTACATGCATTGAGCTCTCTCATTGTCTGTGTAGAGTGTTATACTTGGGAATAT
  AAAGGAGGTGACCAAATCAGTGTGAGGAGGTAGATTTGGCTCCTCTGCTTCTCACGGGACTATTTCCACC
  ACCCCCAGTTAGCACCATTAACTCCTCCTGAGCTCTGATAAGAGAATCAACATTTCTCAATAATTTCCTC
  CACAAATTATTAATGAAAATAAGAATTATTTTGATGGCTCTAACAATGACATTTATATCACATGTTTTCT
  CTGGAGTATTCTATAAGTTTTATGTTAAATCAATAAAGACCACTTTACAAAAGTATTATCAGATGCTTTC
  CTGCACATTAAGGAGAAATCTATAGAACTGAATGAGAACCAACAAGTAAATATTTTTGGTCATTGTAATC
  ACTGTTGGCGTGGGGCCTTTGTCAGAACTAGAATTTGATTATTAACATAGGTGAAAGTTAATCCACTGTG
  ACTTTGCCCATTGTTTAGAAAGAATATTCATAGTTTAATTATGCCTTTTTTGATCAGGCACAGTGGCTCA
  CGCCTGTAATCCTAGCAGTTTGGGAGGCTGAGCCGGGTGGATCGCCTGAGGTCAGGAGTTCAAGACAAGC
  CTGGCCTACATGGTTGAAACCCCATCTCTACTAAAAATACACAAATTAGCTAGGCATGGTGGACTCGCCT
  GTAATCTCACTACACAGGAGGCTGAGGCAGGAGAATCACTTGAACCTGGGAGGCGGATGTTGAAGTGAGC
  TGAGATTGCACCACTGCACTCCAGTCTGGGTGAGAGTGAGACTCAGTCTTAAAAAAATATGCCTTTTTGA
  AGCACGTACATTTTGTAACAAAGAACTGAAGCTCTTATTATATTATTAGTTTTGATTTAATGTTTTCAGC
  CCATCTCCTTTCATATTTCTGGGAGACAGAAAACATGTTTCCCTACACCTCTTGCATTCCATCCTCAACA
  CCCAACTGTCTCGATGCAATG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_017460.3
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases that catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. This enzyme is involved in the metabolism of approximately half the drugs in use today, including acetaminophen, codeine, cyclosporin A, diazepam, erythromycin, and chloroquine. The enzyme also metabolizes some steroids and carcinogens. This gene is part of a cluster of cytochrome P450 genes on chromosome 7q21.1. Previously another CYP3A gene, CYP3A3, was thought to exist; however, it is now thought that this sequence represents a transcript variant of CYP3A4. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2020]'
• Locus ID: 1576