AP1B1 (NM_001127) Human 3' UTR Clone

CAT#: SC212820

3`UTR clone of adaptor-related protein complex 1 beta 1 subunit (AP1B1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: AP1B1
• Synonyms: ADTB1; AP105A; BAM22; CLAPB2
• ACCN: NM_001127
• Insert Size: 1137 bp
• Sequence Data:
  Insert Sequence

  >SC212820 3'UTR clone of NM_001127
  The sequence shown below is from the reference sequence of NM_001127. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACGAGACCATCCTCAAGAACTGAGACCCCGGCCAGCGCCCACCCCAGCCTTCTGCCCGCCCCATCGAGGA
  GGCCCCTCGGGGGCAGCACATCTTCCTCCTCGCAGGAGGGACCAGGCGGGGCTCCAGGCCACTCAGTGGG
  CTCCCTGGTCCTGATGGCAGAACCCACCCGATCCCTGGGGTAGGGCACCACCCCCTCCTGGGGTGAGAGC
  GCAGTGCACTCCCGTGCTCTGGGACACCCCTGCTCCTGTGGCTGTGATGTGGGGTTAAGTGAGGTGGGGA
  CCAAAGGAAACAGAGCCAGAGCAGCCACAGAAGCTGTGCCTGAAGGGTGAGTGTGGAGCTTGCCCCTCCG
  GCTCACAGCCCCGGCAGCCCCTGGCTCCTTGGTCTCTCCGGTTGGTGTTAAAGGGCCCTCCACTGCCACC
  TCTCATGGGATGGACCCTGCCAACCTGGCCTGGGTGTGCAGGGAGGGGTTCCCCTTGGTACCAGGAGAGC
  TGCTCACTTAGGGCCTGGGGCTCAAGGAGCTGTAGGTGCCGGCAGAGGGGCAGAGCTAGGCTGGGAGGAG
  CCCAGGGCCTGCACCACCCACTTGCAACCACCAGGCTGGTGCCCTGCAGCTGTGCCAGTTGGGCCACAGC
  CTCCCGAGTGCTGACCCACATGGTCAAGACCAGGCAGAAGCTCCCAGAGCCCCTGTCTCGGGCTCCCCAC
  CGACTGGCAGCTGCACTATCCCCAACTCCCCACTTCTGCCCCAAGGGTGGTCACTGCCTGTGATACATTC
  CTCAGTGTCGCCTCTGAGCCCAGGCCTCCTGCCACAAAGACTGGGCCGAGAGATGGGGCTGGGTAAGCGG
  GTGCGCCTCCTGTTTGGGTTTCTTCGGGGTTTCTCTGTAGTGTCTGCTGCCCTCCTCCTGGCCTCCCAGG
  TTTCAGTTGTGTCTGACAGAGCATTAGGTTTTCCTGTTACTGCTGTGTAATAATAAAGAAAGATTCGGCT
  TTTGGCAATCACGGACACTTTTTTCCCCTCCACCGAATGTCAGGATTGAAAGCTGCCTCCGAGTGGTTGG
  GGATGGTTTTCTGACCCTACGGTAACTAGATCTAATATATATCATCAGAAAATATATATTGTATGTTTAC
  TCTTATTTTCAGAAAGA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001127.3
• Summary: 'Adaptor protein complex 1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors. This complex is a heterotetramer composed of two large, one medium, and one small adaptin subunit. The protein encoded by this gene serves as one of the large subunits of this complex and is a member of the adaptin protein family. This gene is a candidate meningioma gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]'
• Locus ID: 162