CDC6 (NM_001254) Human 3' UTR Clone

CAT#: SC212842

3`UTR clone of cell division cycle 6 homolog (S. cerevisiae) (CDC6) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CDC6
• Synonyms: CDC18L; HsCDC6; HsCDC18; MGORS5
• ACCN: NM_001254
• Insert Size: 1166 bp
• Sequence Data:
  Insert Sequence

  >SC212842 3'UTR clone of NM_001254
  The sequence shown below is from the reference sequence of NM_001254. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCTTAGCTACTGGATTGCCTTAAATTCTTCTCTTACACCCCACCCGAAAGTATTCAGCTGGCATTTAGAG
  AGCTACAGTCTTCATTTTAGTGCTTTACACATTCGGGCCTGAAAACAAATATGACCTTTTTTACTTGAAG
  CCAATGAATTTTAATCTATAGATTCTTTAATATTAGCACAGAATAATATCTTTGGGTCTTACTATTTTTA
  CCCATAAAAGTGACCAGGTAGACCCTTTTTAATTACATTCACTACTTCTACCACTTGTGTATCTCTAGCC
  AATGTGCTTGCAAGTGTACAGATCTGTGTAGAGGAATGTGTGTATATTTACCTCTTCGTTTGCTCAAACA
  TGAGTGGGTATTTTTTTGTTTGTTTTTTTTGTTGTTGTTGTTTTTGAGGCGCGTCTCACCCTGTTGCCCA
  GGCTGGAGTGCAATGGCGCGTTCTCTGCTCACTACAGCACCCGCTTCCCAGGTTGAAGTGATTCTCTTGC
  CTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCCCACCACCGCGCCCAGCTAATTTTTTAATTTTTAGTA
  GAGACAGGGTTTTACCATGTTGGCCAGGCTGGTCTTGAACTCCTGACCCTCAAGTGATCTGCCCACCTTG
  GCCTCCCTAAGTGCTGGGATTATAGGCGTGAGCCACCATGCTCAGCCATTAAGGTATTTTGTTAAGAACT
  TTAAGTTTAGGGTAAGAAGAATGAAAATGATCCAGAAAAATGCAAGCAAGTCCACATGGAGATTTGGAGG
  ACACTGGTTAAAGAATTTATTTCTTTGTATAGTATACTATGTTCATGGTGCAGATACTACAACATTGTGG
  CATTTTAGACTCGTTGAGTTTCTTGGGCACTCCCAAGGGCGTTGGGGTCATAAGGAGACTATAACTCTAC
  AGATTGTGAATATATTTATTTTCAAGTTGCATTCTTTGTCTTTTTAAGCAATCAGATTTCAAGAGAGCTC
  AAGCTTTCAGAAGTCAATGTGAAAATTCCTTCCTAGGCTGTCCCACAGTCTTTGCTGCCCTTAGATGAAG
  CCACTTGTTTCAAGATGACTACTTTGGGGTTGGGTTTTCATCTAAACACATTTTTCCAGTCTTATTAGAT
  AAATTAGTCCATATGGTTGGTTAATCAAGAGCCTTCTGGGTTTGGT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001254.3
• Summary: 'The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. [provided by RefSeq, Jul 2008]'
• Locus ID: 990