Calpain 2 (CAPN2) (NM_001146068) Human 3' UTR Clone

CAT#: SC212877

3`UTR clone of calpain 2 (m/II) large subunit (CAPN2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CAPN2
• Synonyms: CANP2; CANPL2; CANPml; mCANP
• ACCN: NM_001146068
• Insert Size: 1176 bp
• Sequence Data:
  Insert Sequence

  >SC212877 3'UTR clone of NM_001146068
  The sequence shown below is from the reference sequence of NM_001146068. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGACCTTATCTCTTGGCTCTGTTTCTCAGTACTTTGAAGTTATAACTAATCTGCCTGAAGACTTCTCATG
  ATGGAAAATCAGCCAAGGACTAAGCTTCCATAGAAATACACTTTGTATCTGGACCTCAAAATTATGGGAA
  CATTTACTTAAACGGATGATCATAGCTGAAAATAATGATACTGTCAATTTGAGATAGCAGAAGTTTCACA
  CATCAAAGTAAAAGATTTGCATATCATTATACTAAATGCAAATGAGTCGCTTAACCCTTGACAAGGTCAA
  AGAAAGCTTTAAATCTGTAAATAGTATACACTTTTTACTTTTACACACTTTCCTGTTCATAGCAATATTA
  AATCAGGAAAAAAAAATGCAGGGAGGTATTTAACAGCTGAGCAAAAACATTGAGTCGCTCTCAAAGGACA
  CGAGGCCCTTGGCAGGGAATATTTAAAGCAACTTCAAGTTTAAAATGCAGCTGTTGATTCTACCAAACAA
  CAGTCCAAGATTACCATTTCCCATGAGCCAACTGGGAAACATGGTATATCATGAAGTAATCTTGTCAAGG
  CATCTGGAGAGTCCAGGAGAGAAGACTCACCTCTGTCGCTTGGGTTAAACAAGAGACAGGTTTTGTAGAA
  TATTGATTGGTAATAGTAAATCGTTCTCCTTACAATCAAGTTCTTGACCCTATTCGGCCTTATACATCTG
  GTCTTACAAAGACCAAAGGGATCCTGCGCTTGATCAACTGAACCAGTATGCCAAAACCAGGCATCCAATT
  TGTAAACCAATTATGATAAAGGACAAAATAAGCTGTTTGCCACCTCAAAACTTTATGAACTTCACCACCA
  CTAGTGTCTGTCCATGGAGTTAGAGGGGACATCACTTAGAAGTTCTTATAGAAAGGACACAAGTTTGTTT
  CCTGGCTTTACCTTGGGAAAATGCTAGCAACATTATAGAAATTTTGCCTTGTTGCCTTATCTTCTTCCAA
  ATGTACTGTTAAATAAAAATAAAGGGTTACCCCATGCAATCACACCATGCCATGTTTTCCTTCCTGGAGG
  GCAGCCCCACAGGACGGTTTATGAGCACACAATTATAGCTTGTTTCTACTTTAACAAGGTATGCTGCCTC
  TGTAAATTCATGTATTCAAAGGAAAAGACACCTTGCCTATAATTAAAATGTGGAAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001146068.1
• Summary: 'The calpains, calcium-activated neutral proteases, are nonlysosomal, intracellular cysteine proteases. The mammalian calpains include ubiquitous, stomach-specific, and muscle-specific proteins. The ubiquitous enzymes consist of heterodimers with distinct large, catalytic subunits associated with a common small, regulatory subunit. This gene encodes the large subunit of the ubiquitous enzyme, calpain 2. Multiple heterogeneous transcriptional start sites in the 5' UTR have been reported. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
• Locus ID: 824