SGSH (NM_000199) Human 3' UTR Clone

CAT#: SC212936

3`UTR clone of N-sulfoglucosamine sulfohydrolase (SGSH) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SGSH
• Synonyms: HSS; MPS3A; SFMD
• ACCN: NM_000199
• Insert Size: 1185 bp
• Sequence Data:
  Insert Sequence

  >SC212936 3'UTR clone of NM_000199
  The sequence shown below is from the reference sequence of NM_000199. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCTCCACAATGAGCTGTGACCATCCCAGGAGGCCTGTGCACACATCCCAGGCATGTCCCAGACACATCCC
  ACACGTGTCCGTGTGGCCGGCCAGCCTGGGGAGTAGTGGCAACAGCCCTTCCGTCCACACTCCCATCCAA
  GGAGGGTTCTTCCTTCCTGTGGGGTCACTCTTGCCATTGCCTGGAGGGGGACCAGAGCATGTGACCAGAG
  CATGTGCCCAGCCCCTCCACCACCAGGGGCACTGCCGTCATGGCAGGGGACACAGTTGTCCTTGTGTCTG
  AACCATGTCCCAGCACGGGAATTCTAGACATACGTGGTCTGCGGACAGGGCAGCGCCCCCAGCCCATGAC
  AAGGGAGTCTTGTTTTCTGGCTTGGTTTGGGGACCTGCAAATGGGAGGCCTGAGGCCCTCTTCAGGCTTT
  GGCAGCCACAGATACTTCTGAACCCTTCACAGAGAGCAGGCAGGGGCTTCGGTGCCGCGTGGGCAGTACG
  CAGGTCCCACCGACACTCACCTGGGAGCACGGCGCCTGGCTCTTACCAGCGTCTGGCCTAGAGGAAGCCT
  TTGAGCGACCTTTGGGCAGGTTTCTGCTTCTTCTGTTTTGCCCCATGGTCAAGTCCCTGTTCCCCAGGCA
  GGTTTCAGCTGATTGGCAGCAGGCTCCCTGAGTGATGAGCTTGAACCTGTGGTGTTTCTGGGCAGAAGCT
  TATCTTTTTTGAGAGTGTCCGAAGATGAAGGCATGGCGATGCCCGTCCTCTGGCTTGGGTTAATTCTTCG
  GTGACACTGGCATTGCTGGGTGGTGATGCCCGTCCTCTGGCTTGGGTTAATTCTTCGGTGACACTGGCGT
  TGCTGGGTGGCAATGCCCATCCTCTGCCTTGGGTTAATTCTTCGGTGACACTGGCGTTGCTGGGTGGCGA
  TGCCCGTCCTCTGGCTTGGGTTAATTCTTGGATGACGTCGGCGTTGCTGGGAGAATGTGCCGTTCCTGCC
  CTGCCTCCACCCACCTCGGGAGCAGAAGCCCGGCCTGGACACCCCTCGGCCTGGACACCCCTCGAAGGAG
  AGGGCGCTTCCTTGAGTAGGTGGGCTCCCCTTGCCCTTCCCTCCCTATCACTCCATACTGGGGTGGGCTG
  GAGGAGGCCACAGGCCAGCTATTGTAAAAGCTTTTTATTTTAGTAAAATATACAGAAGTTCTTTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000199.3
• Summary: 'This gene encodes the enzyme sulfamidase; one of several enzymes involved in the lysosomal degradation of heparan sulfate. Mutations in this gene are associated with the lysosomal storage disease mucopolysaccaridosis IIIA, also known as Sanfilippo syndrome A, which results from impaired degradation of heparan sulfate. Transcripts of varying sizes have been reported but their biological validity has not been determined. [provided by RefSeq, Jun 2017]'
• Locus ID: 6448