IDI1 (NM_004508) Human 3' UTR Clone

CAT#: SC212966

3`UTR clone of isopentenyl-diphosphate delta isomerase 1 (IDI1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: IDI1
• Synonyms: IPP1; IPPI1
• ACCN: NM_004508
• Insert Size: 1172 bp
• Sequence Data:
  Insert Sequence

  >SC212966 3'UTR clone of NM_004508
  The sequence shown below is from the reference sequence of NM_004508. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGTTTGTTGACCATGAGAAAATATACAGAATGTGAATATGTAGGTAAATGATTACAGAAAAATTTATCT
  GCTTAACAAACTTAGAATGACTTTTTCCTTTTAAATTTAGTTCTATCATTAATTTATCATTAAATTTAGT
  TCTATCATTTGGTACTATCATTAATGTATTATATACACTGATACTTTAAAACTTGTGTGGAAAAAACTAA
  CTTATAATTTTGTATCACACACCCTGGATATGTGTTCTGTTTCTAAGCGACATTTGTGAGAGATTATTGT
  AAAATGAGAGCGAGCAAATAAAACTTAATTTAATCTTTGCAGATACATACTTATGGGAAATTTGAACAAA
  TGAGTGAAACTCTGTTTTTAGTAGGCTGTGATAAACATTTCCGGAGCACTTGCAGAGGACTTGCTATTTG
  CCAGGTGCTTTATGTATCATTAAATTTTTCTCATAGTTCAGAAAAATGTGCAAAGGAAACTATTGTCTCG
  CTCCTTCAAAACAGTCTTAATTAACTTTCATATTAGCAGATTAAACTAGCAGAGCAGGTTCAAGGGAAAT
  TAAATGATATGGACCCTAATTTGTATCATTCTGAGTTGATTGTGTGGTTTATTCATTCTGGAAACATGTT
  GATACTTACAGTCAGCCACTGCTTTTGATAAGTGATATTGATTAGGTTGAATCTTCTTGTAAATAGTATT
  TACCAGTTAGCAAAGTCTGTGTTTTCAGAATTACAGTGAGCACAGAGGTGTTCATAAAATGGGAATTGAG
  TCCCACTCGGTAAGAGTTGCTTAAACTTGACACTGTTGACATTTGGGCTGGATAAAACCCCTGTGGTGGG
  GTCTGTGCTGTGCATTGCAGGATGGTGAGCAGCGTCCCTCTCATGTGACACCCACAGTTATGCCGGATGT
  TGCCAGATGCCCCTAGGGGACAGAGTCAACCCCCAACTGAGGACCACTGTCCTACAGAGTCAGGAAATAT
  TGTAGGGAGAAAAAAATAACAACAACAAAGGCCTGTGTTAATGTTAAATAGATGAGATTATGGAATGTGT
  ATATTAATGTTAAAAATTGTACCTTGATCAATGTACTTTTTATAAACTTGCCATAGATATCTCAGATTTG
  AAACCTCAAGACAGATTTATTATTCTTAAATGCTGTATGATAATGAAGAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004508.2
• Summary: 'IDI1 encodes a peroxisomally-localized enzyme that catalyzes the interconversion of isopentenyl diphosphate (IPP) to its highly electrophilic isomer, dimethylallyl diphosphate (DMAPP), which are the substrates for the successive reaction that results in the synthesis of farnesyl diphosphate and, ultimately, cholesterol. It has been shown in peroxisomal deficiency diseases such as Zellweger syndrome and neonatal adrenoleukodystrophy that there is reduction in IPP isomerase activity. [provided by RefSeq, Jul 2008]'
• Locus ID: 3422