CD11b (ITGAM) (NM_001145808) Human 3' UTR Clone

CAT#: SC213025

3`UTR clone of integrin alpha M (complement component 3 receptor 3 subunit) (ITGAM) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ITGAM
• Synonyms: CD11B; CR3A; MAC-1; MAC1A; MO1A; SLEB6
• ACCN: NM_001145808
• Insert Size: 1227 bp
• Sequence Data:
  Insert Sequence

  >SC213025 3'UTR clone of NM_001145808
  The sequence shown below is from the reference sequence of NM_001145808. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGACATGATGAGTGAAGGGGGTCCCCCGGGGGCCGAACCCCAGTAGCGGCTCCTTCCCGACAGAGCTGCC
  TCTCGGTGGCCAGCAGGACTCTGCCCAGACCACACGTAGCCCCCAGGCTGCTGGACACGTCGGACAGCGA
  AGTATCCCCGACAGGACGGGCTTGGGCTTCCATTTGTGTGTGTGCAAGTGTGTATGTGCGTGTGTGCAAG
  TGTCTGTGTGCAAGTGTGTGCACATGTGTGCGTGTGCGTGCATGTGCACTTGCACGCCCATGTGTGAGTG
  TGTGCAAGTATGTGAGTGTGTCCAAGTGTGTGTGCGTGTGTCCATGTGTGTGCAAGTGTGTGCATGTGTG
  CGAGTGTGTGCATGTGTGTGCTCAGGGGCGTGTGGCTCACGTGTGTGACTCAGATGTCTCTGGCGTGTGG
  GTAGGTGACGGCAGCGTAGCCTCTCCGGCAGAAGGGAACTGCCTGGGCTCCCTTGTGCGTGGGTGAAGCC
  GCTGCTGGGTTTTCCTCCGGGAGAGGGGACGGTCAATCCTGTGGGTGAAGACAGAGGGAAACACAGCAGC
  TTCTCTCCACTGAAAGAAGTGGGACTTCCCGTCGCCTGCGAGCCTGCGGCCTGCTGGAGCCTGCGCAGCT
  TGGATGGAGACTCCATGAGAAGCCGTGGGTGGAACCAGGAACCTCCTCCACACCAGCGCTGATGCCCAAT
  AAAGATGCCCACTGAGGAATGATGAAGCTTCCTTTCTGGATTCATTTATTATTTCAATGTGACTTTAATT
  TTTTGGATGGATAAGCTTGTCTATGGTACAAAAATCACAAGGCATTCAAGTGTACAGTGAAAAGTCTCCC
  TTTCCAGATATTCAAGTCACCTCCTTAAAGGTAGTCAAGATTGTGTTTTGAGGTTTCCTTCAGACAGATT
  CCAGGCGATGTGCAAGTGTATGCACGTGTGCACACACACCACACATACACACACACAAGCTTTTTTACAC
  AAATGGTAGCATACTTTATATTGGTCTGTATCTTGCTTTTTTTCACCAATATTTCTCAGACATCGGTTCA
  TATTAAGACATAAATTACTTTTTCATTCTTTTATACCGCTGCATAGTATTCCATTGTGTGAGTGTACCAT
  AATGTATTTAACCAGTCTTCTTTTGATATACTATTTTCATTCTCTTGTTATTGCATCAATGCTGAGTTAA
  TAAATCAAATATATGTCATTTTTGCATATATGTAAGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001145808.1
• Summary: 'This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]'
• Locus ID: 3684