HIF-1 alpha (HIF1A) (NM_001530) Human 3' UTR Clone

CAT#: SC213108

3`UTR clone of hypoxia inducible factor 1 alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HIF1A
• Synonyms: bHLHe78; HIF-1-alpha; HIF-1A; HIF-1alpha; HIF1; HIF1-ALPHA; MOP1; PASD8
• ACCN: NM_001530
• Insert Size: 1213
• Sequence Data:
  Insert Sequence

  >SC213108 3'UTR clone of NM_001530
  The sequence shown below is from the reference sequence of NM_001530. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGTGAAGAATTACTCAGAGCTTTGGATCAAGTTAACTGAGCTTTTTCTTAATTTCATTCCTTTTTTTGGA
  CACTGGTGGCTCATTACCTAAAGCAGTCTATTTATATTTTCTACATCTAATTTTAGAAGCCTGGCTACAA
  TACTGCACAAACTTGGTTAGTTCAATTTTGATCCCCTTTCTACTTAATTTACATTAATGCTCTTTTTTAG
  TATGTTCTTTAATGCTGGATCACAGACAGCTCATTTTCTCAGTTTTTTGGTATTTAAACCATTGCATTGC
  AGTAGCATCATTTTAAAAAATGCACCTTTTTATTTATTTATTTTTGGCTAGGGAGTTTATCCCTTTTTCG
  AATTATTTTTAAGAAGATGCCAATATAATTTTTGTAAGAAGGCAGTAACCTTTCATCATGATCATAGGCA
  GTTGAAAAATTTTTACACCTTTTTTTTCACATTTTACATAAATAATAATGCTTTGCCAGCAGTACGTGGT
  AGCCACAATTGCACAATATATTTTCTTAAAAAATACCAGCAGTTACTCATGGAATATATTCTGCGTTTAT
  AAAACTAGTTTTTAAGAAGAAATTTTTTTTGGCCTATGAAATTGTTAAACCTGGAACATGACATTGTTAA
  TCATATAATAATGATTCTTAAATGCTGTATGGTTTATTATTTAAATGGGTAAAGCCATTTACATAATATA
  GAAAGATATGCATATATCTAGAAGGTATGTGGCATTTATTTGGATAAAATTCTCAATTCAGAGAAATCAT
  CTGATGTTTCTATAGTCACTTTGCCAGCTCAAAAGAAAACAATACCCTATGTAGTTGTGGAAGTTTATGC
  TAATATTGTGTAACTGATATTAAACCTAAATGTTCTGCCTACCCTGTTGGTATAAAGATATTTTGAGCAG
  ACTGTAAACAAGAAAAAAAAAATCATGCATTCTTAGCAAAATTGCCTAGTATGTTAATTTGCTCAAAATA
  CAATGTTTGATTTTATGCACTTTGTCGCTATTAACATCCTTTTTTTCATGTAGATTTCAATAATTGAGTA
  ATTTTAGAAGCATTATTTTAGGAATATATAGTTGTCACAGTAAATATCTTGTTTTTTCTATGTACATTGT
  ACAAATTTTTCATTCCTTTTGCTCTTTGTGGTTGGATCTAACACTAACTGTATTGTTTTGTTACATCAAA
  TAAACATCTTCTGTGGACCAGGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001530.3
• Summary: This gene encodes the alpha subunit of transcription factor hypoxia-inducible factor-1 (HIF-1), which is a heterodimer composed of an alpha and a beta subunit. HIF-1 functions as a master regulator of cellular and systemic homeostatic response to hypoxia by activating transcription of many genes, including those involved in energy metabolism, angiogenesis, apoptosis, and other genes whose protein products increase oxygen delivery or facilitate metabolic adaptation to hypoxia. HIF-1 thus plays an essential role in embryonic vascularization, tumor angiogenesis and pathophysiology of ischemic disease. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jul 2011]
• Locus ID: 3091