SHIP (INPP5D) (NM_005541) Human 3' UTR Clone

CAT#: SC213169

3`UTR clone of inositol polyphosphate-5-phosphatase 145kDa (INPP5D) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: INPP5D
• Synonyms: hp51CN; p150Ship; SHIP; SHIP-1; SHIP1; SIP-145
• ACCN: NM_005541
• Insert Size: 1194 bp
• Sequence Data:
  Insert Sequence

  >SC213169 3'UTR clone of NM_005541
  The sequence shown below is from the reference sequence of NM_005541. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGGCAGGACTGCCATGCAGTGAAGCCCTCAGTGAGCTGCCACTGAGTCGGGAGCCCAGAGGAACGGCGTG
  AAGCCACTGGACCCTCTCCCGGGACCTCCTGCTGGCTCCTCCTGCCCAGCTTCCTATGCAAGGCTTTGTG
  TTTTCAGGAAAGGGCCTAGCTTCTGTGTGGCCCACAGAGTTCACTGCCTGTGAGACTTAGCACCAAGTGC
  TGAGGCTGGAAGAAAAACGCACACCAGACGGGCAACAAACAGTCTGGGTCCCCAGCTCGCTCTTGGTACT
  TGGGACCCCAGTGCCTCGTTGAGGGCGCCATTCTGAAGAAAGGAACTGCAGCGCCGATTTGAGGGTGGAG
  ATATAGATAATAATAATATTAATAATAATAATGGCCACATGGATCGAACACTCATGATGTGCCAAGTGCT
  GTGCTAAGTGCTTTACGAACATTCGTCATATCAGGATGACCTCGAGAGCTGAGGCTCTAGCCACCTAAAA
  CCACGTGCCCAAACCCACCAGTTTAAAACGGTGTGTGTTCGGAGGGGTGAAAGCATTAAGAAGCCCAGTG
  CCCTCCTGGAGTGAGACAAGGGCTCGGCCTTAAGGAGCTGAAGAGTCTGGGTAGCTTGTTTAGGGTACAA
  GAAGCCTGTTCTGTCCAGCTTCAGTGACACAAGCTGCTTTAGCTAAAGTCCCGCGGGTTCCGGCATGGCT
  AGGCTGAGAGCAGGGATCTACCTGGCTTCTCAGTTCTTTGGTTGGAAGGAGCAGGAAATCAGCTCCTATT
  CTCCAGTGGAGAGATCTGGCCTCAGCTTGGGCTAGAGATGCCAAGGCCTGTGCCAGGTTCCCTGTGCCCT
  CCTCGAGGTGGGCAGCCATCACCAGCCACAGTTAAGCCAAGCCCCCCAACATGTATTCCATCGTGCTGGT
  AGAAGAGTCTTTGCTGTTGCTCCCGAAAGCCGTGCTCTCCAGCCTGGCTGCCAGGGAGGGTGGGCCTCTT
  GGTTCCAGGCTCTTGAAATAGTGCAGCCTTTTCTTCCTATCTCTGTGGCTTTCAGCTCTGCTTCCTTGGT
  TATTAGGAGAATAGATGGGTGATGTCTTTCCTTATGTTGCTTTTTCAACATAGCAGAATTAATGTAGGGA
  GCTAAATCCAGTGGTGTGTGTGAATGCAGAAGGGAATGCACCCCACATTCCCATGATGGAAGTCTGCGTA
  ACCA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005541.3
• Summary: 'This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. The protein is also partly localized to the nucleus, where it may be involved in nuclear inositol phosphate signaling processes. Overall, the protein functions as a negative regulator of myeloid cell proliferation and survival. Mutations in this gene are associated with defects and cancers of the immune system. Deficiencies in the encoded protein, SHIP1, have been associated with Inflammatory Bowel Disease types such as Crohn's Disease and Ulcerative Colitis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2020]'
• Locus ID: 3635