DUSP6 (NM_001946) Human 3' UTR Clone

CAT#: SC213264

3`UTR clone of dual specificity phosphatase 6 (DUSP6) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DUSP6
• Synonyms: HH19; MKP3; PYST1
• ACCN: NM_001946
• Insert Size: 1199 bp
• Sequence Data:
  Insert Sequence

  >SC213264 3'UTR clone of NM_001946
  The sequence shown below is from the reference sequence of NM_001946. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATACCAGGTGGACTCTCTGCAATCTACGTGAAAGACCCCACACCCCTCCTTGCTGGAATGTGTCTGGCCC
  TTCAGCAGTTTCTCTTGGCAGCATCAGCTGGGCTGCTTTCTTTGTGTGTGGCCCCAGGTGTCAAAATGAC
  ACCAGCTGTCTGTACTAGACAAGGTTACCAAGTGCGGAATTGGTTAATACTAACAGAGAGATTTGCTCCA
  TTCTCTTTGGAATAACAGGACATGCTGTATAGATACAGGCAGTAGGTTTGCTCTGTACCCATGTGTACAG
  CCTACCCATGCAGGGACTGGGATTCGAGGACTTCCAGGCGCATAGGGTAGAACCAAATGATAGGGTAGGA
  GCATGTGTTCTTTAGGGCCTTGTAAGGCTGTTTCCTTTTGCATCTGGAACTGACTATATAATTGTCTTCA
  ATGAAGACTAATTCAATTTTGCATATAGAGGAGCCAAAGAGAGATTTCAGCTCTGTATTTGTGGTATCAG
  TTTGGAAAAAAAAATCTGATACTCCATTTGATTATTGTAAATATTTGATCTTGAATCACTTGACAGTGTT
  TGTTTGAATTGTGTTTGTTTTTTCCTTTGATGGGCTTAAAAGAAATTATCCAAAGGGAGAAAGAGCAGTA
  TGCCACTTCTTAAAACAGAACAAAACAAAAAAAGAAAATTGTGCTCTTTTCTAATCCAAAGGGTATATTT
  GCAGCATGCTTGACTTTACCAATTCTGATGACATCTTTACGGACACTATTATCACTAAGACCTTGTTATG
  GCGAAGTCTTTAGTCTTTTTCATGTATTTTCCTCATGATTTTTTCTCTTTATGTAGTTTGACTATGCCTT
  ACCTTTGTAAATATTTTTGCTTGTGTTGTCGCAAAGGGGATAATCTGGGAAAGACACCAAATCATGGGCT
  CACTTTAAAAAAAGAAAGAATAAAAAAACCTTCAGCTGTGCTAAACAGTATATTACCTCTGTATAAAATT
  CTTCAGGGAGTGTCACCTCAAATGCAATACTTTGGGTTGGTTTCTTTCCTTTAAAAAAATTTGTATAAAA
  CTGGAAGTGTGTGTGTGTGAGCATGGGTACCCATTTGATAAGAGAAATGCATTTGATTGTGAAGAAGGGA
  GAGTTAAATTCTCCATTATGTTCGTGGTGTAAAGTTTAGAGCTGGAATTTATTATAAGAATGTAAAACCT
  TAAATTATT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001946.2
• Summary: 'The protein encoded by this gene is a member of the dual specificity protein phosphatase subfamily. These phosphatases inactivate their target kinases by dephosphorylating both the phosphoserine/threonine and phosphotyrosine residues. They negatively regulate members of the mitogen-activated protein (MAP) kinase superfamily (MAPK/ERK, SAPK/JNK, p38), which are associated with cellular proliferation and differentiation. Different members of the family of dual specificity phosphatases show distinct substrate specificities for various MAP kinases, different tissue distribution and subcellular localization, and different modes of inducibility of their expression by extracellular stimuli. This gene product inactivates ERK2, is expressed in a variety of tissues with the highest levels in heart and pancreas, and unlike most other members of this family, is localized in the cytoplasm. Mutations in this gene have been associated with congenital hypogonadotropic hypogonadism. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]'
• Locus ID: 1848