SNAP25 (NM_130811) Human 3' UTR Clone

CAT#: SC213291

3`UTR clone of synaptosomal-associated protein 25kDa (SNAP25) transcript variant 2 for miRNA target validation

Reconstitution Protocol

USD 560.00

4 Weeks*

Size
    • 10 ug

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Specifications

Product Data
Vector pMirTarget
Species Human
Transfection Reporter RFP
Assay Reporter Luciferase
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Symbol SNAP25
Synonyms bA416N4.2; CMS18; dJ1068F16.2; RIC-4; RIC4; SEC9; SNAP; SNAP-25; SUP
ACCN NM_130811
Insert Size 1224 bp
Sequence Data
>SC213291 3'UTR clone of NM_130811
The sequence shown below is from the reference sequence of NM_130811. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon


CAATTGGCAGAGCTCAGAATTCAAGCGATCGC

CGTGCAACAAAGATGCTGGGAAGTGGTTAAGTGTGCCCACCCGTGTTCTCCTCCAAATGCTGTCGGGCAA
GATAGCTCCTTCATGCTTTTCTCATGGTATTATCTAGTAGGTCTGCACACATAACACACATCAGTCCACC
CCCATTGTGAATGTTGTCCTGTGTCATCTGTCAGCTTCCCAACAATACTTTGTGTCTTTTGTTCTCTCTT
GGTCTCTTTCTTTCCAAAGGTTGTACATAGTGGTCATTTGGTGGCTCTAACTCCTTGATGTCTTGAGTTT
CATTTTTCATTTTCTCTCCTCGGTGGCATTTGCTGAATAACAACAATTTAGGAATGCTCAATGTGCTGTT
GATTCTTTCAATCCACAGTATTGTTCTTGTAAAACTGTGACATTCCACAGAGTTACTGCCACGGTCCTTT
GAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTCCTCATGGCTGTTATCTGTCTTTATGA
TTTCATGATTAGACAATGTGGAATTACATAACAGGCATTGCACTAAAAGTGATGTGATTTATGCATTTAT
GCATGAGAACTAAATAGATTTTTAGATTCCTACTTAAACAAAAACTTTCCATGACAGTAGCATACTGATG
AGACAACACACACACACACAAAACAACAGCAACAACAACAGAACAACAACAAAGCATGCTCAGTATTGAG
ACACTGTCAAGATTAAGTTATACCAGCAAAAGTGCAGTAGTGTCACTTTTTTCCTGTCAATATATAGAGA
CTTCTAAATCATAATCATCCTTTTTTAAAAAAAAGAATTTTAAAAAAGATGGATTTGACACACTCACCAT
TTAATCATTTCCAGCAAAATATATGTTTGGCTGAAATTATGTCAAATGGATGTAATATAGGGTTTGTTTG
CTGCTTTTGATGGCTACGTTTTGGAGAGAGCAATCTTGCTGTGAAACAGTGTGGATGTAAATTTTATAAG
GCTGACTCTTACTAACCACCATTTCCCCTGTGGTTTGTTATCAGTACAATTCTTTGTTGCTTAATCTAGA
GCTATGCACACCAAATTGCTGAGATGTTTAGTAGCTGATAAAGAAACCTTTTAAAAAAATAATATAAATG
AATGAAATATAAACTGTGAGATAAATATCATTATAGCATGTAATATTAAATTCCTCCTGTCTCCTCTGTC
AGTTTGTGAAGTGATTGACATTTTGTAGCTAGTT

ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
Restriction Sites SgfI-MluI     
OTI Disclaimer Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.
Reference Data
RefSeq NM_130811.1
Summary 'Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]'
Locus ID 6616

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.