POLR2J2 (NM_032959) Human 3' UTR Clone

CAT#: SC213366

3`UTR clone of polymerase (RNA) II (DNA directed) polypeptide J2 (POLR2J2) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: POLR2J2
• Synonyms: HRPB11B; POLR2J3; RPB11b1; RPB11b2
• ACCN: NM_032959
• Insert Size: 1226
• Sequence Data:
  Insert Sequence

  >SC213366 3'UTR clone of NM_032959
  The sequence shown below is from the reference sequence of NM_032959. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTTCGCCTTCTGCCGTGATTGTCAGTTTCCTGAGGCCTCCCCAGCCACGCTTCCTGTACAGCCTGCAGAA
  CTCTGCCCCAGAGCACATCAGCTATGTGCCCCAGCTCTCAAACGACACCTTGGCGGGGAGGCTCACCCTG
  TCCACCTTCACGCTGGAGCAGCCTCTAGGCCAGTTCAGCAGCCACAACATCTCTGACTTGGATACCATCT
  GGCTGGTGGTGGCCCTCAGCAACGCCACCCAGAGCTTCACGGCCCCACGGACAAACCAGGACATCCCTGC
  TCCTGCCAACTTCTCCCAGAGGGGCTACTATCTCACACTGAGGGCCAACCGGGTGCTGTACCAGACCAGA
  GGCCAGCTCCATGTCCTCCGCGTCGGCAATGATACCCACTGCCAACCAACAAAAATTGGCTGCAACCATC
  CCCTACCAGGACCCGGCCCCTACAGGGTGAAGTTCCTGGTGATGAATGACGAAGGACCCGTGGCTGAAAC
  CAAGTGGTCCAGCGACACTCGCCTGCAGCAAGCCCAGGCACTTCGGGCTGTCCCCGGCCCCCAGAGCCCG
  GGCACCGTGGTCATCATCGCCATCCTGTCTATCCTCCTGGCCGTCCTCCTCACGGTCCTCCTGGCTGTGC
  TCATATACACCTGCTTCAACAGCTGCAGGAGCACTTCCCTATCAGGCCCAGAGGAGGCAGGGAGTGTGAG
  AAGATACACCACGCACCTCGCGTTCAGCACTCCTGCCGAGGGGGCTTCCTGAGGGGTTCCAGAGGGGCCC
  ACGTGTCCCTCCACCTCCTCCCTGGCCCAGGCTGCAGAGCCTGAGCTGGGACACGCCCTGAAGCTTCTGG
  ACCCTGAGAGAGATTGTTTCTTTTCTTTACTATTTTTTTTTTTTTTTTTGAGACGGAGTCTCACTCTGTC
  GCCAGGCTGGCGCGATCTTGGCTCACTGCAACCTCTGCCTCACGGGTTCAAGCGATTTTCCTGCTTCAGC
  CTCCTGAGTAGCTGGGACTACAGGCACACGCCACCATGCCCAGCTCATTTTTGTATTTTTAGTAGAGATG
  GGGTTTCACCATGTTGGCCAGGATGGCCTCCATCTCTTGACCTTGTGATCCGCCCGACTCGGCCTCCCAA
  AATGCTGGGATTACAGGCGTGAGCCATCAAGTCTGGCGAGAGAGATTGTTTCTAGATGAGGGTGGGGGCG
  GGTGTCCTTAGCCCAAAGCTTGTGCCAGTCTCTATC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_032959.4
• Summary: This gene is a member of the RNA polymerase II subunit 11 gene family, which includes three genes in a cluster on chromosome 7q22.1 and a pseudogene on chromosome 7p13. The founding member of this family, DNA directed RNA polymerase II polypeptide J, has been shown to encode a subunit of RNA polymerase II, the polymerase responsible for synthesizing messenger RNA in eukaryotes. This locus produces multiple, alternatively spliced transcripts that potentially express isoforms with distinct C-termini compared to DNA directed RNA polymerase II polypeptide J. Most or all variants are spliced to include additional non-coding exons at the 3' end which makes them candidates for nonsense-mediated decay (NMD). Consequently, it is not known if this locus expresses a protein or proteins in vivo. [provided by RefSeq, Jul 2008]
• Locus ID: 246721