MMP2 (NM_004530) Human 3' UTR Clone

CAT#: SC213498

3`UTR clone of matrix metallopeptidase 2 (gelatinase A 72kDa gelatinase 72kDa type IV collagenase) (MMP2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MMP2
• Synonyms: CLG4; CLG4A; MMP-2; MMP-II; MONA; TBE-1
• ACCN: NM_004530
• Insert Size: 1243
• Sequence Data:
  Insert Sequence

  >SC213498 3'UTR clone of NM_004530
  The sequence shown below is from the reference sequence of NM_004530. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAAGCATCAAATCCGACTGGCTAGGCTGCTGAGCTGGCCCTGGCTCCCACAGGCCCTTCCTCTCCACTG
  CCTTCGATACACCGGGCCTGGAGAACTAGAGAAGGACCCGGAGGGGCCTGGCAGCCGTGCCTTCAGCTCT
  ACAGCTAATCAGCATTCTCACTCCTACCTGGTAATTTAAGATTCCAGAGAGTGGCTCCTCCCGGTGCCCA
  AGAATAGATGCTGACTGTACTCCTCCCAGGCGCCCCTTCCCCCTCCAATCCCACCAACCCTCAGAGCCAC
  CCCTAAAGAGATACTTTGATATTTTCAACGCAGCCCTGCTTTGGGCTGCCCTGGTGCTGCCACACTTCAG
  GCTCTTCTCCTTTCACAACCTTCTGTGGCTCACAGAACCCTTGGAGCCAATGGAGACTGTCTCAAGAGGG
  CACTGGTGGCCCGACAGCCTGGCACAGGGCAGTGGGACAGGGCATGGCCAGGTGGCCACTCCAGACCCCT
  GGCTTTTCACTGCTGGCTGCCTTAGAACCTTTCTTACATTAGCAGTTTGCTTTGTATGCACTTTGTTTTT
  TTCTTTGGGTCTTGTTTTTTTTTTCCACTTAGAAATTGCATTTCCTGACAGAAGGACTCAGGTTGTCTGA
  AGTCACTGCACAGTGCATCTCAGCCCACATAGTGATGGTTCCCCTGTTCACTCTACTTAGCATGTCCCTA
  CCGAGTCTCTTCTCCACTGGATGGAGGAAAACCAAGCCGTGGCTTCCCGCTCAGCCCTCCCTGCCCCTCC
  CTTCAACCATTCCCCATGGGAAATGTCAACAAGTATGAATAAAGACACCTACTGAGTGGCCGTGTTTGCC
  ATCTGTTTTAGCAGAGCCTAGACAAGGGCCACAGACCCAGCCAGAAGCGGAAACTTAAAAAGTCCGAATC
  TCTGCTCCCTGCAGGGCACAGGTGATGGTGTCTGCTGGAAAGGTCAGAGCTTCCAAAGTAAACAGCAAGA
  GAACCTCAGGGAGAGTAAGCTCTAGTCCCTCTGTCCTGTAGAAAGAGCCCTGAAGAATCAGCAATTTTGT
  TGCTTTATTGTGGCATCTGTTCGAGGTTTGCTTCCTCTTTAAGTCTGTTTCTTCATTAGCAATCATATCA
  GTTTTAATGCTACTACTAACAATGAACAGTAACAATAATATCCCCCTCAATTAATAGAGTGCTTTCTATG
  TGCAAGGCACTTTTCACGTGTCACCTATTTTAACCTTTCCAACCACATAAATA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_004530.4
• Summary: This gene is a member of the matrix metalloproteinase (MMP) gene family, that are zinc-dependent enzymes capable of cleaving components of the extracellular matrix and molecules involved in signal transduction. The protein encoded by this gene is a gelatinase A, type IV collagenase, that contains three fibronectin type II repeats in its catalytic site that allow binding of denatured type IV and V collagen and elastin. Unlike most MMP family members, activation of this protein can occur on the cell membrane. This enzyme can be activated extracellularly by proteases, or, intracellulary by its S-glutathiolation with no requirement for proteolytical removal of the pro-domain. This protein is thought to be involved in multiple pathways including roles in the nervous system, endometrial menstrual breakdown, regulation of vascularization, and metastasis. Mutations in this gene have been associated with Winchester syndrome and Nodulosis-Arthropathy-Osteolysis (NAO) syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]
• Locus ID: 4313