Nck beta (NCK2) (NM_001004722) Human 3' UTR Clone

CAT#: SC213685

3`UTR clone of NCK adaptor protein 2 (NCK2) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: NCK2
• Synonyms: GRB4; NCKbeta
• ACCN: NM_001004722
• Insert Size: 1281 bp
• Sequence Data:
  Insert Sequence

  >SC213685 3'UTR clone of NM_001004722
  The sequence shown below is from the reference sequence of NM_001004722. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAACCTGAAGGACACACTAGCCCAGCGACTTCTCCGTGTCCCTTAAAGCGTCAGGGAAGAACAAACACTT
  CAAGGTGCAGCTCGTGGACAATGTCTACTGCATTGGGCAGCGGCGCTTCCACACCATGGACGAGCTGGTG
  GAACACTACAAAAAGGCGCCCATCTTCACCAGCGAGCACGGGGAGAAGCTCTACCTCGTCAGGGCCCTGC
  AGTGACGGCGCCCCGGCCCCACACTCGCCTCCCGGGCCCCACGGTGGAGCTGCCCGCCCGGCCTTGTGGC
  AGAGGCTCCTCCCGCGGGGACGGCCCCGACGGCTTCTCTGCGAGTCTCTCTTTATGTTCAGGTCGCTTGG
  TCGGTTCGTCTCCCATTTGCCATCCAGGCCTCACACCCACACTCGAGCCCACCCGGCCGGCCAGCTTTAG
  AGGAGGGGAGGAGCAGGGCGAGTTCACATTATTCCTTTTCCATCGGAAGTGGCGCTCGTGCATTCAACTC
  GTTCCCGCTCATGGAACCCCTCTTTAAAAAGACGCAGGGCACCTGTGAGCGCAGGAGCGAGCCTAAGGCC
  ACCCAGCGGCAGCGCCCGTGTCCTGGGCACTCAGCGTGCTGGGCAGAGCAGGTGCGATGGCCCCAGTCCT
  AGCAGCCCTCGCCCATGTCCTGTGCCCTTACATGGCTCCCGGACTGTGCAGGGAGCCGATACGTTTGCTG
  ATAGCAATACTGGAACCACCGGGTGCGATGGCAGTGAGGAGACTGCCCAGTGCCTTTGGGGCTGTGCTTG
  CAATAAAGAATTTCCTGGAAAGGCAGTCTGCAAAAGAGGGAACCGGTGACTCAGAAAGACAGATGTTTTG
  GTAATTTACCCCAAATGTGCCATCCACATAGTGCTTTTTCCTCTTGCCCTTCGGCTTGTTTGAATCTCAC
  AATTATGTATTTAATTCTCAAAGAAATATGTATCTGTAGCCGTTTGTTGACACTAATACAGATGATTAAG
  GAAAACAGCTGATCTTTGGGGAAGGGAGCTACCAACACTTTATACACACACACACGTGCACACACACACA
  CACACACACTATATATATATATATTATTTACAGGGAAATTTTTCAGGGTTTACAAAAGAGTATGTGATTG
  GTAGTAAGAGACACACAGAATGTTTATGAAGAAATTGCATTTTCTTTTTCCTTTACATTTGAACTTCTTT
  ATAGTTTAAATATAACGTCTTGAGATGGCACATTCCTACGATTGAAGAAGGGGTCTTGAGATCCCCTAAA
  CTTGCATACCCAGTTTTTTGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001004722.1
• Summary: This gene encodes a member of the NCK family of adaptor proteins. The protein contains three SH3 domains and one SH2 domain. The protein has no known catalytic function but has been shown to bind and recruit various proteins involved in the regulation of receptor protein tyrosine kinases. It is through these regulatory activities that this protein is believed to be involved in cytoskeletal reorganization. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
• Locus ID: 8440