Tissue Factor (F3) (NM_001993) Human 3' UTR Clone

CAT#: SC213705

3`UTR clone of coagulation factor III (thromboplastin tissue factor) (F3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: F3
• Synonyms: CD142; TF; TFA
• ACCN: NM_001993
• Insert Size: 1294 bp
• Sequence Data:
  Insert Sequence

  >SC213705 3'UTR clone of NM_001993
  The sequence shown below is from the reference sequence of NM_001993. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGAGCTGGAAGGAGAACTCCCCACTGAATGTTTCATAAAGGAAGCACTGTTGGAGCTACTGCAAATGCTA
  TATTGCACTGTGACCGAGAACTTTTAAGAGGATAGAATACATGGAAACGCAAATGAGTATTTCGGAGCAT
  GAAGACCCTGGAGTTCAAAAAACTCTTGATATGACCTGTTATTACCATTAGCATTCTGGTTTTGACATCA
  GCATTAGTCACTTTGAAATGTAACGAATGGTACTACAACCAATTCCAAGTTTTAATTTTTAACACCATGG
  CACCTTTTGCACATAACATGCTTTAGATTATATATTCCGCACTCAAGGAGTAACCAGGTCGTCCAAGCAA
  AAACAAATGGGAAAATGTCTTAAAAAATCCTGGGTGGACTTTTGAAAAGCTTTTTTTTTTTTTTTTTTTT
  TTTTGAGACGGAGTCTTGCTCTGTTGCCCAGGCTGGAGTGCAGTAGCACGATCTCGGCTCACTGCACCCT
  CCGTCTCTCGGGTTCAAGCAATTGTCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGCGCACTACC
  ACGCCAAGCTAATTTTTGTATTTTTTAGTAGAGATGGGGTTTCACCATCTTGGCCAGGCTGGTCTTGAAT
  TCCTGACCTCAGGTGATCCACCCACCTTGGCCTCCCAAAGTGCTAGTATTATGGGCGTGAACCACCATGC
  CCAGCCGAAAAGCTTTTGAGGGGCTGACTTCAATCCATGTAGGAAAGTAAAATGGAAGGAAATTGGGTGC
  ATTTCTAGGACTTTTCTAACATATGTCTATAATATAGTGTTTAGGTTCTTTTTTTTTTCAGGAATACATT
  TGGAAATTCAAAACAATTGGCAAACTTTGTATTAATGTGTTAAGTGCAGGAGACATTGGTATTCTGGGCA
  CCTTCCTAATATGCTTTACAATCTGCACTTTAACTGACTTAAGTGGCATTAAACATTTGAGAGCTAACTA
  TATTTTTATAAGACTACTATACAAACTACAGAGTTTATGATTTAAGGTACTTAAAGCTTCTATGGTTGAC
  ATTGTATATATAATTTTTTAAAAAGGTTTTCTATATGGGGATTTTCTATTTATGTAGGTAATATTGTTCT
  ATTTGTATATATTGAGATAATTTATTTAATATACTTTAAATAAAGGTGACTGGGAATTGTTACTGTTGTA
  CTTATTCTATCTTCCATTTATTATTTATGTACAATTTGGTGTTTGTATTAGCTCTACTACAGTAAATGAC
  TGTAAAATTGTCAGTGGCTTACAACAACGTATCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001993.3
• Summary: 'This gene encodes coagulation factor III which is a cell surface glycoprotein. This factor enables cells to initiate the blood coagulation cascades, and it functions as the high-affinity receptor for the coagulation factor VII. The resulting complex provides a catalytic event that is responsible for initiation of the coagulation protease cascades by specific limited proteolysis. Unlike the other cofactors of these protease cascades, which circulate as nonfunctional precursors, this factor is a potent initiator that is fully functional when expressed on cell surfaces, for example, on monocytes. There are 3 distinct domains of this factor: extracellular, transmembrane, and cytoplasmic. Platelets and monocytes have been shown to express this coagulation factor under procoagulatory and proinflammatory stimuli, and a major role in HIV-associated coagulopathy has been described. Platelet-dependent monocyte expression of coagulation factor III has been described to be associated with Coronavirus Disease 2019 (COVID-19) severity and mortality. This protein is the only one in the coagulation pathway for which a congenital deficiency has not been described. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Aug 2020]'
• Locus ID: 2152