Lysosomal acid lipase (LIPA) (NM_000235) Human 3' UTR Clone

CAT#: SC213717

3`UTR clone of lipase A lysosomal acid cholesterol esterase (LIPA) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: LIPA
• Synonyms: CESD; LAL
• ACCN: NM_000235
• Insert Size: 1276 bp
• Sequence Data:
  Insert Sequence

  >SC213717 3'UTR clone of NM_000235
  The sequence shown below is from the reference sequence of NM_000235. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATCTAATGAGGAAATATCAGTGAAAGCTGGACTTGAGCTGTGTACCACCAAGTCAATGATTATGTCATGT
  GAAAATGTGTTTGCTTCATTTCTGTAAAACACTTGTTTTTCTTTCCCAGGTCTTTTGTTTTTTTATATCC
  AAGAAAATGATAACTTTGAAGATGCCCAGTTCACTCTAGTTTCAATTAGAAACATACTAGCTATTTTTTC
  TTTAATTAGGGCTGGAATAGGAAGCCAGTGTCTCAACCATAGTATTGTCTCTTTAAGTCTTTTAAATATC
  ACTGATGTGTAAAAAGGTCATTATATCCATTCTGTTTTTAAAATTTAAAATATATTGACTTTTTGCCCTT
  CATAGGACAAAGTAATATATGTGTTGGAATTTTAAAATTGTGTTGTCATTGGTAAATCTGTCACTGACTT
  AAGCGAGGTATAAAAGTACGCAGTTTTCATGTCCTTGCCTTAAAGAGCTCTCTAGTCTAACGGTCTTGTA
  GTTAGAGATCTAAATGACATTTTATCATGTTTTCCTGCAGCAGGTGCATAGTCAAATCCAGAAATATCAC
  AGCTGTGCCAGTAATAAGGATGCTAACAATTAATTTTATCAAACCTAACTGTGACAGCTGTGATTTGACA
  CGTTTTAATTGCTCAGGTTAAATGAAATAGTTTTCCGGCGTCTTCAAAAACAAATTGCACTGATAAAACA
  AAAACAAAAGTATGTTTTAAATGCTTTGAAGACTGATACACTCAACCATCTATATTCATGAGCTCTCAAT
  TTCATGGCAGGCCATAGTTCTACTTATCTGAGAAGCAAATCCCTGTGGAGACTATACCACTATTTTTTCT
  GAGATTAATGTACTCTTGGAGCCCGCTACTGTCGTTATTGATCACATCTGTGTGAAGCCAAAGCCCCGTG
  GTTGCCCATGAGAAGTGTCCTTGTTCATTTTCACCCAAATGAAGTGTGAACGTGATGTTTTCGGATGCAA
  ACTCAGCTCAGGGATTCATTTTGTGTCTTAGTTTTATATGCATCCTTATTTTTAATACACCTGCTTCACG
  TCCCTATGTTGGGAAGTCCATATTTGTCTGCTTTTCTTGCAGCATCATTTCCTTACAATACTGTCCGGTG
  GACAAAATGACAATTGATATGTTTTTCTGATATAATTACTTTAGCTGCACTAACAGTACAATGCTTGTTA
  ATGGTTAATATAGGCAGGGCGAATACTACTTTGTAACTTTTAAAGTCTTAAACTTTTCAATAAAATTGAG
  TGAGACTTATAGGCCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000235.2
• Summary: 'This gene encodes lipase A, the lysosomal acid lipase (also known as cholesterol ester hydrolase). This enzyme functions in the lysosome to catalyze the hydrolysis of cholesteryl esters and triglycerides. Mutations in this gene can result in Wolman disease and cholesteryl ester storage disease. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2014]'
• Locus ID: 3988