PLOD2 (NM_000935) Human 3' UTR Clone

CAT#: SC213728

3`UTR clone of procollagen-lysine 2-oxoglutarate 5-dioxygenase 2 (PLOD2) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLOD2
• Synonyms: BRKS2; LH2; TLH
• ACCN: NM_000935
• Insert Size: 1292 bp
• Sequence Data:
  Insert Sequence

  >SC213728 3'UTR clone of NM_000935
  The sequence shown below is from the reference sequence of NM_000935. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGAACAAGATACATTGCAGTGTCATTTATAGATCCCTAAGTTATTTACTTTTCATTGAATTGAAATTTAT
  TTTGGATGAATGACTGGCATGAACACGTCTTTGAAGTTGTGGCTGAGAAGATGAGAGGAATATTTAAATA
  ACATCAACAGAACAACTTCACTTTGGGCCAAACATTTGAAAAACTTTTTATAAAAAATTGTTTGATATTT
  CTTAATGTCTGCTCTGAGCCTTAAAACACAGATTGAAGAAGAAAAGAAAGAAAAAACTTAAATATTTATT
  TCTATGCTTTGTTGCCTCTGAGAATAATGACAATTTATGAATTTGTGTTTCAAATTGATAAAATATTTAG
  GTACAAATAACAAGACTAATAATATTTTCTTATTTAAAAAAAGCATGGGAAGATTTTTATTTATCAAAAT
  ATAGAGGAAATGTAGACAAAATGGATATAAATGAAAATTACCATGTTGTAAAACCTTGAAAATCAGATTC
  TAACTGGATTTGTATGCAACTAAGTATTTTTCTGAACACCTATGCAGGTCTTATTTACAGTAGTTACTAA
  GGGAACACACAAAGAATTACACAACGTTTTCCTCAAGAAAATGGTACAAAACACAACCGAGGAGCGTATA
  CAGTTGAAAACATTTTTGTTTTGATTGGAAGGCAGATTATTTTATATTAGTATTAAAAATCAAACCCTAT
  GTTTCTTTCAGATGAATCTTCCAAAGTGGATTATATTAAGCAGGTATTAGATTTAGGAAAACCTTTCCAT
  TTCTTAAAGTATTATCAAGTGTCAAGATCAGCAAGTGTCCTTAAGTCAAACAGGTTTTTTTTGTTGTTGT
  TTTTGCTTTGTTTCCTTTTTTAGAAAGTTCTAGAAAATAGGAAAACGAAAAATTTCATTGAGATGAGTAG
  TGCATTTAATTATTTTTTAAAAAACTTTTTAAGTACTTGAATTTTATATCAGGAAAACAAAGTTGTTGAG
  CCTTGCTTCTTCCGTTTTGCCCTTTGTCTCGCTCCTTATTCTTTTTTTGGGGGGAGGGTTATTTGCTTTT
  TTATCTTCCTGGCATAATTTCCATTTTATTCTTCTGAGTGTCTATGTTAACTTCCCTCTATCCCGCTTAT
  AAAAAAATTCTCCAACAAAAATACTTGTTGACTTGATGTTTTATCACTTCTCTAAGTAAGGTTGAAATAT
  CCTTATTGTAGCTACTGTTTTTAATGTAAAGGTTAAACTTGAAAAGAAATTCTTAATCACGGTGCCAAAA
  TTCATTTTCTAACACCATGTGTTAGAAAATTA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000935.2
• Summary: 'The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'
• Locus ID: 5352