HCCS (NM_005333) Human 3' UTR Clone

CAT#: SC213740

3`UTR clone of holocytochrome c synthase (cytochrome c heme-lyase) (HCCS) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: HCCS
• Synonyms: CCHL; LSDMCA1; MCOPS7; MLS
• ACCN: NM_005333
• Insert Size: 1303 bp
• Sequence Data:
  Insert Sequence

  >SC213740 3'UTR clone of NM_005333
  The sequence shown below is from the reference sequence of NM_005333. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGAAAGTCGCTTGGTGGCGTTGGACCTCGTAAAGCACTGTTTCAGATGGAAAAATATAAACTATTTTTTT
  CTGAGCGATACATTAAACTATTTTCCCCAGATTGAATTGCACTCATGATGTAATGGGAACTTCAAGTGGG
  TAATCACACTTTTTCCTTCACTTAACGAAGGATACTATGCACTGTTCACTTTTGACTTGTGTTGTACCTT
  GCAGTTTATAGCAGATCATTTTGTTTTCTTCCTACTTAGCTTAAGTGGGAAGCAGTCTCTCAGTTTTTCC
  TTTACCTGCAGTATTGTCTGTATATTTCATCTCGAAAAGCCTGAACAACCTCTGAAAAAGAGCTTTTAAA
  AAGTGATGAAAGTTGCTTTTTTTTCTTACTATTTTCTCAGGAATACTCCAGAGATGTAAAGCCTGAGAGT
  CCATGGTGATGCTCAAGAGCTTTCATCTGGCCAAATATTTTAACTAAAGCTGTTCATTATAAATAAGAGG
  TGATTATACCTTTTTTAAAAAAAGTTACCATTATTTCTAAAGCCCCTATGGTATGATTGTGACCTTTAAC
  AAAATGCAAAGGTATACACTGGAAAGGTACTCAGCAAAATTCTCTCCAAATTCTGATTTATATGAATTTC
  TTGGACTGGATAGAGTACTTTCAACTATACCTTCCTTTGGAACATACCTTTTAAAAAATGTTCCCTTTAA
  ATCCTGTTATTCTAATATGTAACTCTGCTCCCAACAAACAGGTCTCCTCATTGTCCTTGGCATTTGACAA
  GTTATCCCAACTTCGAATCTCATTTCTTCCTTTGCTGGCAGCCTCCACCCTTCCCCACATCAGTCGGCCT
  AATCCAAACCTTGAAAGCCTGGCTTAAGTCCCAGTATCAGTGATGGTCCCCCTCTAAAACATGATTTTAT
  ATTTGGCTGAGTGTTAAAATTCACAAGATGAGCATTTTTTTCCCAGATGGAGAACGCTTCAACTCAGTCT
  AGATTTTTTTTGTTTGTTTTAAGGAAGCTCTAAGTTTGCCATTGAAACTTTTTGTACCAGTAATCCATAA
  ACTGGTTGTTCTATTTATAGCAGCATGTTACAAATGACTGCTTTTTTTTTTTAACTGTCTATACTATTAA
  ATCCTTTAATTTATCCTGAATGCTGTAGTGTTAAATTAGTGGATGAAATTGGCAGTCTGTGGGTGATTAA
  TTTGGTATGGATTTGTCAGTTTTGTGTGGAGTTTAAGAAATAGAACAGCCTTAAACTCTACATTTCAAAA
  TACTTGTATATTTTAAAATAAAGCTGATTAGTTCACAGGTTTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005333.3
• Summary: 'The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010]'
• Locus ID: 3052