CYP7A1 (NM_000780) Human 3' UTR Clone

CAT#: SC213775

3`UTR clone of cytochrome P450 family 7 subfamily A polypeptide 1 (CYP7A1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CYP7A1
• Synonyms: CP7A; CYP7; CYPVII
• ACCN: NM_000780
• Insert Size: 1334 bp
• Sequence Data:
  Insert Sequence

  >SC213775 3'UTR clone of NM_000780
  The sequence shown below is from the reference sequence of NM_000780. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGCCATTGAATGATATTGAATTTAAATATAAATTCAAGCATTTGTGAATACATGGCTGGAATAAGAGGAC
  ACTAGATGATATTACAGGACTGCAGAACACCCTCACCACACAGTCCCTTTGGACAAATGCATTTAGTGGT
  GGTAGAAATGATTCACCAGGTCCAATGTTGTTCACCAGTGCTTGCTTGTGAATCTTAACATTTTGGTGAC
  AGTTTCCAGATGCTATCACAGACTCTGCTAGTGAAAAGAACTAGTTTCTAGGAGCACAATAATTTGTTTT
  CATTTGTATAAGTCCATGAATGTTCATATAGCCAGGGATTGAAGTTTATTATTTTCAAAGGAAAACACCT
  TTATTTTATTTTTTTTCAAAATGAAGATACACATTACAGCCAGGTGTGGTAGCAGGCACCTGTAGTCTTA
  GCTACTCGAGAGGCCAAAGAAGGAGGATGGCTTGAGCCCAGGAGTTCAAGACCAGCCTGGACAGCTTAGT
  GAGATCCCGTCTCCGAAGAAAAGATATGTATTCTAATTGGCAGATTGTTTTTTCCTAAGGAAACTGCTTT
  ATTTTTATAAAACTGCCTGACAATTATGAAAAAATGTTCAAATTCACGTTCTAGTGAAACTGCATTATTT
  GTTGACTAGATGGTGGGGTTCTTCGGGTGTGATCATATATCATAAAGGATATTTCAAATGATTATGATTA
  GTTATGTCTTTTAATAAAAAGGAAATATTTTTCAACTTCTTCTATATCCAAAATTCAGGGCTTTAAACAT
  GATTATCTTGATTTCCCAAAAACACTAAAGGTGGTTTTATTTTCCCTTCATGTTTTAACTTATTGTTGCT
  GAAAACTCTATGTCCGGCTTTAACTATCTTCTCTATATTTTTATTTCATTCACATTAATGAGAAGAGTTT
  TCTCAGAGATTAAAAAAGGTAGTTTTTCTGTCATTGTTAAATACACATTATCACTGAAAAAATGTAGCTT
  TTATGTGATATGTTTTAAAGTTAAAACTGGATGGAAATAGCCATTTGGAAGCTTTGGTTATGAAACATGT
  GGAGTGTATTAAGTGCAGCTTGACATTATGTTTTATTTAAATGCTTTTTATCGCTAAATGACTTGCAGAT
  GAAAAAAACTAAGGTGACTCGAGTGTTTAAATGCTGTGTACAACAATGCTTTGATAAAATATTTTAAGTA
  TGAGTTATCAGCTCTATGTCAATTGATATTTCTGTGTAGTATTTATATTTAAATTATATTTACCTTTTTG
  CTTATTTTACAAATATTAAGAAAATATTCTAACATTTGATAATTTTGAAATGATTCATCTTTCAGAAATA
  AAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000780.3
• Summary: 'This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum membrane protein catalyzes the first reaction in the cholesterol catabolic pathway in the liver, which converts cholesterol to bile acids. This reaction is the rate limiting step and the major site of regulation of bile acid synthesis, which is the primary mechanism for the removal of cholesterol from the body. Polymorphisms in the promoter of this gene are associated with defects in bile acid synthesis. [provided by RefSeq, Feb 2010]'
• Locus ID: 1581