CHRNA3 (NM_000743) Human 3' UTR Clone

CAT#: SC213784

3`UTR clone of cholinergic receptor nicotinic alpha 3 (CHRNA3) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CHRNA3
• Synonyms: BAIPRCK; LNCR2; NACHRA3; PAOD2
• ACCN: NM_000743
• Insert Size: 1344 bp
• Sequence Data:
  Insert Sequence

  >SC213784 3'UTR clone of NM_000743
  The sequence shown below is from the reference sequence of NM_000743. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCAGGATTGTTTCTGCAACCCCTGATGGCCAGGGAAGATGCATAAGCACTAAGCTGTGTGCCTGCCTGGG
  AGACTTCCTTGTGTCAGGGCAGGAGGAGGCTGCTTCCTAGTAAGAACGTACTTTCTGTTATCAAGCTACC
  AGCTTTGTTTTTGGCATTTCGAGGTTTACTTATTTTCCACTTATCTTGGAATCATGCAAAAAAAAAAATG
  TCAAGAGTATTTATTACCGATAAATGAACATTTAACTAGCCTTTTTGGTATGGTAAAGAGATGTCAAAAT
  GTGATTCTATGTGATTAGTATGCTATGCTATGGAATATACATGTAAAAATGTTTCCTTTTAGTTGTTGAA
  ACAAAACTGGATAGAAAAATGCTGTTCAGAAATATGAAAAGTCATTCAGTTATCACTACAGATCTCCCAG
  TAATTTTTCTTATTTAGCCCATAATCTCTTTGAAGGTTTATACTAATTCAGCAATCCCCCATCGTTACCC
  ATTTCTTACCATGCATTTCTCGTTCTTTACTGGGTCTAAAGGGCTATGCCTCCATTTCAGAGAGCTTCAA
  CTACTTCTCTTGCATACTTCTAAATTATACCATGAGAAATCATGCCTAGTTATTCATTGTTAATATAACT
  GTCTTAGTACACCATAAACTGGGTGGATTATAAACAACAGAAACTTCTCAGTTTTGGAGGTTGGGAGGTC
  CAAGGTCAAGGCACCAGCAAATTTGGTGTCTGGTGAGGGTCCTCTTCCTCAAAGGGTGCCTTCTAGCTGT
  GTCCTCACATGACTGAAGGGACTAGCTATCTCTGTGGGGTCTATTTTATAAGGGCACTAACCCCATTCAT
  GAGAGCAGAGCCCCCATGGCCTAATCACCTTTCCAAGGCCCCACCTTCTATCTAAGACAATCACGCTGGG
  AATAGGTTTCAACATATGAATTGGGGGAGGACACATTTGGACCACAGCATGAACCTTTAGAACAGGGTTT
  CTCAGCCTTAGCACTATGGACATTTTGGGCTGGATAAATATGTGTTGGTACAGAATGGGGGTATCCTGTG
  CATTGTAGGATCTTTAGCAGTACCCTAGCCTCAACTCACTAGATGCCAATGACATACCTTGCTTCTTCAC
  CAGTTATGATAACCAAGAATGTCTCCATTGTTAAATGTTCCCTTAGGAGCAAAATTGCCCCTGGTTGAGA
  AACATTGCTTTAGACAAATTGTTAAGAGTATCATGTACTACACTTCTGAAACTTAACGTGATCATCACCA
  CTGACAGATGATTCACAGAGACTGTTTGAATCTTGTCTCACTAGTTTTTCCTGTGCAAAAATAAAATGGA
  CAGAATTGCAGCCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000743.4
• Summary: 'This locus encodes a member of the nicotinic acetylcholine receptor family of proteins. Members of this family of proteins form pentameric complexes comprised of both alpha and beta subunits. This locus encodes an alpha-type subunit, as it contains characteristic adjacent cysteine residues. The encoded protein is a ligand-gated ion channel that likely plays a role in neurotransmission. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer. Alternatively spliced transcript variants have been described. [provided by RefSeq, Nov 2009]'
• Locus ID: 1136