NMDAR2B (GRIN2B) (NM_000834) Human 3' UTR Clone

CAT#: SC213840

3`UTR clone of glutamate receptor ionotropic N-methyl D-aspartate 2B (GRIN2B) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GRIN2B
• Synonyms: EIEE27; GluN2B; hNR3; MRD6; NMDAR2B; NR2B; NR3
• ACCN: NM_000834
• Insert Size: 1315 bp
• Sequence Data:
  Insert Sequence

  >SC213840 3'UTR clone of NM_000834
  The sequence shown below is from the reference sequence of NM_000834. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTATTGAGTCTGATGTCTGAGTGAGGGAACAGAGAGGTTAAGGTGGGTACGGGAGGGTAAGGCTGTGGGT
  CGCGTGATGCGCATGTCACGGAGGGTGACGGGGGTGAACTTGGTTCCCATTTGCTCCTTTCTTGTTTTAA
  TTTATTTATGGGATCCTGGAGTTCTGGTTCCTACTGGGGGCAACCCTGGTGACCAGCACCATCTCTCCTC
  CTTTTCACAGTTCTCTCCTTCTTCCCCCCGCTGTCAGCCATTCCTGTTCCCATGAGATGATGCCATGGGC
  CCTCTCAGCAGGGGAGGGTAGAGCGGAGAAAGGAAGGGCTGCATGCGGGCTTCCTCCTGGTGTGGAAGAG
  CTCCTTGATATCCTCTTTGAGTGAAGCTGGGAGAACCAAAAAGAGGCTATGTGAGCACAAAGGTAGCTTT
  TCCCAAACTGATCTTTTCATTTAGGTGAGGAAGCAAAAGCATCTATGTGAGACCATTTAGCACACTGCTT
  GTGAAAGGAAAGAGGCTCTGGCTAAATTCATGCTGCTTAGATGACATCTGTCTAGGAATCATGTGCCAAG
  CAGAGGTTGGGAGGCCATTTGTGTTTATATATAAGCCAAAAAATGCTTGCTTCAACCCCATGAGACTCGA
  TAGTGGTGGTGAACAGAACAAAAGGTCATTGGTGGCAGAGTGGATTCTTGAACAAACTGGAAAGTACGTT
  ATGATAGTGTCCCACGGTGCCTTGGGGACAAGAGCAGGTGGATTGTGCGTGCATGTGTGTTCATGCACAC
  TTGCACCCATGTGTAGTCAGGTGCCTCAAGAGAAGGCAACCTTGACTCTTTCTATTGTTTCTTTCAATAT
  CCCCAAGCAGTGTGATTGTTTGGCTTATATACAGACAGAGATGGCCATGTATTACCTGAATTTTGGCTGT
  GTCTCCCTTCATCCTTCTGGAATAAGGAGAATGAAAATTCTTGATAAAGAAGATTCTGTGGTCTAAACAA
  AAAAAGGCGGTGAGCAATCCTGCAAGAACAAGGTACATAAACAAGTCCTCAGTGGTTGGCAATTGTTTCA
  ACCAGTTTGAACCAAGAACTTTCCAGGAAGGCTAAAGGGAAACCGAATTTTCACAGCCATGATTCTTTTG
  CCCACACTTGGGAGCAAAAGATTCTACAAAGCTCTTTTGAGCATTTAGACTCTCGACTGGCCAAGGTTTG
  GGGAAGAACGAAGCCACCTTTGAAGAAGTAAGGAGTCGTGTATGGTAGGGTAAGTGAGAGAGGGGGATGT
  TTCCAATGCTTTGATCCCTTCTTACTTAACCTGAAGCTAGACGAGCAGGCTTCTT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000834.3
• Summary: 'This gene encodes a member of the N-methyl-D-aspartate (NMDA) receptor family within the ionotropic glutamate receptor superfamily. The encoded protein is a subunit of the NMDA receptor ion channel which acts as an agonist binding site for glutamate. The NMDA receptors mediate a slow calcium-permeable component of excitatory synaptic transmission in the central nervous system. The NMDA receptors are heterotetramers of seven genetically encoded, differentially expressed subunits including NR1 (GRIN1), NR2 (GRIN2A, GRIN2B, GRIN2C, or GRIN2D) and NR3 (GRIN3A or GRIN3B). The early expression of this gene in development suggests a role in brain development, circuit formation, synaptic plasticity, and cellular migration and differentiation. Naturally occurring mutations within this gene are associated with neurodevelopmental disorders including autism spectrum disorder, attention deficit hyperactivity disorder, epilepsy, and schizophrenia. [provided by RefSeq, Aug 2017]'
• Locus ID: 2904