DCTD (NM_001921) Human 3' UTR Clone

CAT#: SC213843

3`UTR clone of dCMP deaminase (DCTD) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: DCTD
• Synonyms: MGC111062
• ACCN: NM_001921
• Insert Size: 1334 bp
• Sequence Data:
  Insert Sequence

  >SC213843 3'UTR clone of NM_001921
  The sequence shown below is from the reference sequence of NM_001921. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  AGCAGACCGAGTCAAAAGCTTCAGTGAGTTACATCTCATTCAATCTCCAGAAGATTGGGATTATCGTCTT
  CTAAGAGGTTGCTAATGCCTTTCATCTTGAAGTTACACATAACTTCTTACTAGCCAGTATGGCAAAAGTA
  GGCATCTAAAGAATATAAAGCCTCAAATCTTCCTTACTGTCTCTCTTGTCACATGGAATCTACATGTGTT
  TGAACTATTGCTTTAGGGTTTAAAATAGGGGAGCCTGTGGTGGCCTGGTGCACAGGGCTAGAACGAGAGT
  GCCTCCCCTTCTTGTGTCCTGGCTGGCTGGGATGCTGGTGGCTCTTCAGAGGAGCATCAGCTGTCTGTCA
  TCTGCTGCGATCCGGCAGCCTCTCTTCACTGCTACATGTGCTGGAAGGACAAATAAATAATTGTGGTTGT
  GTTCTTAATGGGGACGAGCAGACACACTGATCTGAACATCTGGCCCAAGTGAAGCATGGCATATAGTGCC
  CTTGGAAGAAAATTAGGCCTCAAATGACAGTAGCATTGAAGTGTTTGCTGCAGAGTTGAGGGAAACCCCC
  AGCCACCCTCCCGGAATCCGAGATAGGGTGGCACATCTGTCCTGACAGACGAGGAGTGTAACTGAACCAG
  GAATATTTCCTCCATTCCTGCTCTCCCACTGCACACAGGGTGGTGGCACATTATCCCTCTGGGGGGTGGG
  GACGCCTGTTGTTTTGGCTCAATTTGGGTTTGTTGGTCACATGGAGCTCTTCCATTTCGTTTAGCTGAAT
  AATGAGTTGTTCCTAGAGGAGACAGCCTGTCTCTCCTTGTTGCCCCCAAAGCCCATGCCCTGCCGTGGTG
  GCAGCTGGGGCTGTGGATGGGAGGGGTCCCCAACATGGATGTGTTGCCCCTCCTCCGCATGCCAACGCAG
  TTCATGTACAAGGCCCCTCTGCAACTGGAGAGAAAATTAATTCCTATCCCGTGAGTGGATTGTGAGAAAT
  TCCACCCACGTGGAGACAGCTTACTGCAGCACTGTTGGTGTTCGGAGCTCTTCTGTGCCCTGGCTCCATG
  CTTTCACCTACACAAGCATCACCTTCCTAATCACCGCGGGGCGGGGAGCGTGTGGCTGTGCCCCTTCTCT
  TTAATCTCATTTAATTTTTATTAAACATGCTCAGTACCTGTGTTGAGAAAAGGCTTTCTTTATCCTAAAG
  ATTATTACCTTTTTAAAGTGCTCTTATATTTTCATGAGTTTTTATTTTGTCTCTGAGATTTTGTATTCCA
  CATTCTAGGGTATTCTGTAATTTGGCTCCTTACCAATATTATTAAAATCTTATTAAAATCTACCCTACCA
  AAGC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001921.2
• Summary: 'The protein encoded by this gene catalyzes the deamination of dCMP to dUMP, the nucleotide substrate for thymidylate synthase. The encoded protein is allosterically activated by dCTP and inhibited by dTTP, and is found as a homohexamer. This protein uses zinc as a cofactor for its activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
• Locus ID: 1635