SAE1 (NM_001145713) Human 3' UTR Clone

CAT#: SC213882

3`UTR clone of SUMO1 activating enzyme subunit 1 (SAE1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SAE1
• Synonyms: AOS1; HSPC140; SUA1; UBLE1A
• ACCN: NM_001145713
• Insert Size: 1346
• Sequence Data:
  Insert Sequence

  >SC213882 3'UTR clone of NM_001145713
  The sequence shown below is from the reference sequence of NM_001145713. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ATGCCCACCTGTATTCCCTGTCCCCTTCCTTCATGAAGGCATCTCCAGGCAAGGAAAACTGAAGTCATTG
  GCCCGATACAAAACATTTCCTGCAACGAAGGAGGTGGTGCCGACGTGCTGCTTCCCATCACCAGCAGCTG
  CTCGACAAGGGGCGCAGGGTGGCTGTCTTTGTTCCAGCACTGTTCAGGCTGCCTGTCATCCCGGGCCTGC
  CAGCTCCCCTGAGTGATGAGCACTTCCAAGCACCCCTCTGCCCTTTCTCTGTCCTTATGCTGTCCCGGCC
  TCGCCAGCCCTCTGGGGCATTGTGGGAGATGCCTGCCAGGAATGAGCAAGCTCTGTTGCTCGGGAGCCTC
  TTGTCACCTTCTTGGACTTATTCCCCACCTGATACCTTATAGAGAAAAGTGTGAATTCAGGTGGAGAGTA
  GGCCCAGGCCCCATGAGGCACCAGTGGAAGCACAGCTCCAAGTTCAGACAGGTGCCCTTAGAGAGGAAAA
  CCATGACAGGCAAATGCATTTCCTCTGGAGTTTGAGACCCTGACAAACAACAGGTGGCATCTGGTGTGCT
  GTTCTTGAGTTTTCGTTTAGGATTAGTTGAGTTCCAGCTGGGTTTTGGGAGAAAGGAGATGCTACCAAGT
  CTTGGATGTTAGGGCGAGACCCTGCAAGTTGAGTATTAGAGAGCTTGTCTTTCAAGGCAGGTTCCTGGGG
  CTTCAGGGCTAGGAGGGAGGAGCCTGCCCTTTTAACAGAACCCCAGTCACATGCGGCTCAAGTCACTCAG
  AGGCTGTTGCATTTCAGGGCTATGTTGGTCCTTTGTTTACCTCCTAAACCACAGCTGTTTGTGTTTCACA
  TATGTTGTGAATTTTCCTTGGTTCTTTTTAAAGGAATGATAATAAAGTTACTTGCTTTAGGATTTGCTTG
  TTTTTCTTCCACTTCAGAAGCTTCTGAGAGGGAATGGGATGATCCTACCAGTTGCCTTTTCAGACCTGAG
  GCTCTAACTCAAGAGATTCCTCCTCTCCCTCACCATTCCTGCCACCATTTTTTCTGGGTGATGCAGCAAG
  AGTTAAATTGTTCACATTCTAGAATGTGTAGAAGCTTCTGGCTCCAGTTGTCTAATGGATAATTCAGCTA
  AATAGTGGCTATTTTCAGTGGCAAGAATTATAATAATAAAGGGAAGTCAAAAGTGATGCTTCATATGCCC
  CAAGTGCCACAGCCGCTACGGGTCAGAGCTTTTATTGGAAGACCAGGTTTGCCTTGATGCAGCCAAAGTG
  CGTTCCAGTTCACCCCACTCTTGCTTGTGTTTCTTCTTTATGTAAATTGTGTGATGTTTCAATAAATCAG
  TCTCTTTCCTATTCTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_001145713.1
• Summary: Posttranslational modification of proteins by the addition of the small protein SUMO (see SUMO1; MIM 601912), or sumoylation, regulates protein structure and intracellular localization. SAE1 and UBA2 (MIM 613295) form a heterodimer that functions as a SUMO-activating enzyme for the sumoylation of proteins (Okuma et al., 1999 [PubMed 9920803]). [supplied by OMIM, Mar 2010]
• Locus ID: 10055