RUNX1T1 (NM_175635) Human 3' UTR Clone

CAT#: SC213895

3`UTR clone of runt-related transcription factor 1; translocated to 1 (cyclin D-related) (RUNX1T1) transcript variant 3 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RUNX1T1
• Synonyms: AML1-MTG8; AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2
• ACCN: NM_175635
• Insert Size: 1347 bp
• Sequence Data:
  Insert Sequence

  >SC213895 3'UTR clone of NM_175635
  The sequence shown below is from the reference sequence of NM_175635. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CTTCCACCATAGAGACAACCCCTCGCTAGACGTGAACTCAGAACTGTCGGAGGAAAGACAACACAACCAA
  CGCGAAACCAATTCCTCATCCTCAGATGCTCAAAGTTGTTTTTTTTGTTTGTTTGTTTATTAGATGAATT
  ATCCTATTTCAGTACTTCAGCAAGAGAGAACCTAACTGTATCTTGAGGTGGTAGTAAAACACAGAGGGCC
  AGTAACGGGTCGTAATGACTTATTGTGGATAACAAAGATATCTTTTCTTTAGAGAACTGAAAAGAGAGCA
  GAGAATATAACATGAAATGATAGATTTGACCTCCTCCCTGTTATTTTCAAGTAGCTGGGATTTTAAACTA
  GATGACCTCATTAACCGATGCTTTACCAAACAGCAAACCAAGAGATTGCTAATTGCTGTTGAAAGCAAAA
  ATGCTAATATTAAAAGTCACAATGTTCTTTATATACAATAATGGAAAAAAAAAAAAGAGGAAAACCCTCA
  AGGGCATGAGCATTGGATACAGCAGTAGACATTTTAACAAGAAGATGAATGGCGTCCGTGGGTTGCTAAC
  TGAACTTTGAAGACCCGCTACAAAACGCGCAGATGTGCAGCAGATTGGAAGGAGACACAGATGTTCGGTT
  TTTTTCCTGTTTCTGAAAAGAAATAATAATATCCAGGTCAACAGAATGAAAAATGAAAGATGATTTGCAA
  TGGGATGTATGAATACAGCAGCAAGAAAAAAAAATGCCATAATACAAAAGGCTCCATATATATATATATA
  TATATATATATACACACACACACACGCACATATACACACACACACACACACACACACACACACACACACA
  CACACACACACACACACAAGTAAGAGACTCAGCCTGCAGTTAATTAGCATTCTGGCAGTTTTGACATCAG
  CCAGCTGCCCTAAATAACCCTTCAACGTTTCTTCACTTTTGCAAGGTTCCACAGAGTAAGACATTGGGTC
  TATTCCAGCTCATTCATTTTATATTGAAAAAAATAATTTTAAAAATGGTGGCTTCAGCTCCAGCCCCTTT
  CCAAAATTTTTCAACCCCACCCTGTTTGGATTTTTAATTAAAAACTAGTAGTTCTCTTGGTGTTAAAACA
  CTTCTGTCCTGTGAGGTTTCCCAATGGTGTTTTTCTTGTAAATGTGTTGGACAAATGTGAAGATGCATTG
  TAGTTTAACCATATGCCCACATTTAGTCTCTTTATTCCTAGTTGGTGAGAAACCTGTATCTTTCTATGCT
  GCTTTTATATCTGTATGTATTAGTGATATTTCTCTAGTAGTTAAAAAAAAAAAAAGGAAAAAAAGACTCT
  TTTTGGTTGTCCTCAAG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_175635.1
• Summary: 'This gene encodes a member of the myeloid translocation gene family which interact with DNA-bound transcription factors and recruit a range of corepressors to facilitate transcriptional repression. The t(8;21)(q22;q22) translocation is one of the most frequent karyotypic abnormalities in acute myeloid leukemia. The translocation produces a chimeric gene made up of the 5'-region of the runt-related transcription factor 1 gene fused to the 3'-region of this gene. The chimeric protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]'
• Locus ID: 862