GLEPP1 (PTPRO) (NM_030668) Human 3' UTR Clone

CAT#: SC213975

3`UTR clone of protein tyrosine phosphatase receptor type O (PTPRO) transcript variant 4 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PTPRO
• Synonyms: GLEPP1; NPHS6; PTP-OC; PTP-U2; PTPROT; PTPU2; R-PTP-O
• ACCN: NM_030668
• Insert Size: 1345 bp
• Sequence Data:
  Insert Sequence

  >SC213975 3'UTR clone of NM_030668
  The sequence shown below is from the reference sequence of NM_030668. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACGAGAATGTTAGCAAGTCCTAGTTCAGAATCCGGAGCAGAGAGGACATGATGTGCGCCCATCCTCCCTT
  GCTTCCAGATTGTTTTAGTGGGCCCTGATGGTCATTTTTCTAAACAGAGGCCCTGCTTTGTAATATGTGG
  CCAAGGAGATAATTTATCTCACAGAAGCACCGGGAAGACTTAGCCTTAAAGAGCCTACAGTGTCCTTTTG
  GACTCTTTCACTTCGGGACATTTAATAATGGACCAAATTCAACAGAACACCAGGAAGGTCAAGACGCTCT
  CCAAAGGGCAGGAAGTACAGCACTTCCGAAGAGTTTAGTTGGCCCTTTGCTGGTTGGGCTGAGTTTTTTA
  TTTTTAAGTGTTTGTTTTTCAGTGCAATAATTTTTGTGTGTGTGTGATTCTTATCAGAAAGTTGAATTGT
  TTTCTGCCTACACCGTTCATCAGCCCCATAACCCAGGAAGGAACAGGCATTGTTAGCATCAGATTATACC
  TCATTATTAAAAGGAGGCATGGCCACACATGAAGAAATGGTCATTCTACTTCAAAGAAATTGAGCCAGCA
  CTATCTGTACTCCAACATTACCGGATCTGGATTGGGGAGGTTGGTCAGGGAAGAGAGGGGTTCTACCCAC
  AGATCAACTGTGTAATCTTTTACTATTCAAGCTATAATTCAGCTTCAAAGTAGAGTAGAAAAAAAATTGT
  CTTAACTGTTCTAGTTCTTGATGGTTTTCTTCCTTATTAACAGTTGGTGTTTCTTCCTTGGCCCTTTTGG
  ACTAATGTTACTGTCCAAGTTCTTTCTCAAGAAACCACATCTGGTTCAGAAGAGTGTCAAGTTGGACTCT
  TTGAACTCTGTTGCTGTCTGAGCAATCGTGGTGCCTAGACTTTGCATTCCTTGTTCTGTTGACCTGCATA
  CATGTGAGAGCTATTTCTTTAAGAACTATATAGGCTGTGAAAACGCACTTTCTTTCCCCCAAAGAGCTGG
  GAATTTATGAAGTTATGGCAATGAACTGCAGCATGCTGGGACAATTATTTGACTACTTTTTTTTGTAATA
  TTGTCAAATGTCTCTATGGATTCTGACAGAGATTTCTTTTTGTTTTGTTATTCTTTTGGTTGTCAGTTTC
  ATTTTAACGAGTGTAACTAGTAACATTTTATTCTTTGGATTTTGTATAATTACAGTACATGATTGTGTAT
  TGTGACATGAATGCTGTCAAAATGACATTGATGGCATTGTGAAGCCTGTTACTTTGTGTCACTTCCTGAT
  AAATAAGAGGTGATGACATGGATATACAACAGAAAACACTTTGAGTTGAAAGTAAACACAAGCTGGCTGC
  TTCCCTGTGGCAACT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_030668.1
• Summary: 'This gene encodes a member of the R3 subtype family of receptor-type protein tyrosine phosphatases. These proteins are localized to the apical surface of polarized cells and may have tissue-specific functions through activation of Src family kinases. This gene contains two distinct promoters, and alternatively spliced transcript variants encoding multiple isoforms have been observed. The encoded proteins may have multiple isoform-specific and tissue-specific functions, including the regulation of osteoclast production and activity, inhibition of cell proliferation and facilitation of apoptosis. This gene is a candidate tumor suppressor, and decreased expression of this gene has been observed in several types of cancer. [provided by RefSeq, May 2011]'
• Locus ID: 5800