KCNJ5 (NM_000890) Human 3' UTR Clone

CAT#: SC214021

3`UTR clone of potassium inwardly-rectifying channel subfamily J member 5 (KCNJ5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: KCNJ5
• Synonyms: CIR; GIRK4; KATP1; KIR3.4; LQT13
• ACCN: NM_000890
• Insert Size: 1357 bp
• Sequence Data:
  Insert Sequence

  >SC214021 3'UTR clone of NM_000890
  The sequence shown below is from the reference sequence of NM_000890. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGGGTGGGTCCAGGGAGGCCAGGGGCTCGGTGTGAGGGGTGCAGCCTCCCTAAGACCTCCTGTCACTGGC
  TTCAGTGAACACAGACACTGCAGAGCCTGGGAGCAGGGGAGGGGAATAGTTGAGTGTGCTGTTTGGGGGC
  TCAGGAGCCATCAAGGCTGTGGGGAGGAACCATAAACCCAGCCCTCACAGCTCCCAGCACAGGGCCTCCC
  TGAGCCAGTGGCATCCTGCCTGGGCCCCCCATGGCAGTGCTGCCTCTTGTAGGTGCTGGCTAAGGGCCAG
  GCCAGGATGAGTTTCCCCATGGTGAATGTTACCGGATGGCATCTGTTCTCTCCATGCCCTGGGTCACTTC
  CTTTTTTGGGTCTCACAGACCCCTCCAGGGGCTGACACCTAGAGAGAACCATCACCTTGTCCCTCATTCC
  TTCCACCCTGAGGCTTGCTGTGGACTCAGAGAGGAGACTTACCTGATGAGAGCTCAAACCTCTAGTCTGG
  GATGAGCTCACAGAGCCCTCATGAGTTAAGATCCATCCATATCACATAGGCAATTCCTTTTAAATCAGAT
  ACCAACGACCCATTACTGAGAGGTACAGAGCGTACAGCCCTTGGTGTTCTCTGCTGTTATCTGCCAAATG
  TGTGTGTTTTTCCTCTGCTGTGTTCTGTGTGTCCAGTGTAGCCTATTGGAGAATCGGTCTCATTAGAGTG
  ACTCTTAGAAGGTGGCCTGGGACTAGACAGCCCCTCTGAGCACATGCATAGTGGCATCAGCAGCTTCCTG
  GCCTACTCCCAATCACACTGTGCCCTGCACCTCCCACCAAGGGGGAACCCCAGCAGCTGGGGCTTTTCGA
  GTGCTTTTTGGCCCAAGAATCTCAGAGTGCTTCTAATCATCACAGTAGTTTTTGTGTTTTGTTTTGTTTT
  GTTTTGTTTTGTTTTGTTTTGTTTTGTTTTTTTCACAATCTCAGCCAGCCTGCGGTGTGACCTGGGGAGA
  AAGTTGAGTGTCAGGGGACATGATGAGCCAGTTGGCCCTGAAACAGGAATGGGGCAAAGAGAAAGGAGAG
  CCAGGAGCATTTAAAAATCAGCATCTGGGCCAGGCAAGATGGCTCACGCCTGTGATCCCAGCATTTTGGG
  AGGCCGAGGCGGGTGGATCATGAGGTCAGGAGATCGAGACCATCCTGGCTAACACGGTGAAACCCCGTCT
  CTACTGAAAATACAAAAAATTAGCCAGGCATGGTGGCAGGTGCCTGTAGTCCCAGCTACTCGGGAAGCTG
  AGGCAGAAGAATGGCATGAACCTGGGAGGTGGAGGTTGCAGTGAGCCAAGATCATGCCACTGCACTCCAG
  CCTGGGCGACAGAGCGAGACTCCATCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000890.3
• Summary: 'This gene encodes an integral membrane protein which belongs to one of seven subfamilies of inward-rectifier potassium channel proteins called potassium channel subfamily J. The encoded protein is a subunit of the potassium channel which is homotetrameric. It is controlled by G-proteins and has a greater tendency to allow potassium to flow into a cell rather than out of a cell. Naturally occurring mutations in this gene are associated with aldosterone-producing adenomas. [provided by RefSeq, Aug 2017]'
• Locus ID: 3762