RFC1 (NM_002913) Human 3' UTR Clone

CAT#: SC214073

3`UTR clone of replication factor C (activator 1) 1 145kDa (RFC1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: RFC1
• Synonyms: A1; CANVAS; MHCBFB; PO-GA; RECC1; RFC; RFC140
• ACCN: NM_002913
• Insert Size: 1350 bp
• Sequence Data:
  Insert Sequence

  >SC214073 3'UTR clone of NM_002913
  The sequence shown below is from the reference sequence of NM_002913. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GATAAGGAGCCCAGAAAAGGAAAAGGAAAAAGTTCGAAGAAATGAAACCATTTTTCACTAGCGACAGCCA
  CTTTTTACTCTCCCTCCTGACCAGTCCAGCTGGTCTAGAGAAAGCCTTGTTTTTTCCCAGAGCAACCATG
  TTTTAGCATAATGGGATGACCTGGTGTCCCATTATAAATAAAGGGTGGTATGGCTAGAAGGGTATGAGCA
  GTAGGCTTATGTACACCTCTTATAGAGGTTGATAGGACTGCTTGGGTCCTCCACTGTCCTCTGTCAATCT
  AGTTAGACGTGCTTCTGAATGACTGTAGAATTGGAACTAGAAACTACACCTGGGCTTTGGAGTCAGATTT
  TAGTTAACAATAATGAGCCTGGGAGCAGTAGTACTAAGGCGTCTTTTGTAGGCTTAAGAATTTATCCTAA
  TGGCCTTTATAGGACCAATGCTGATTTTTTTTAAAGGCAGTTCCTATTATGTGGTGAAATTTTGTAAATA
  AATGATTATACAAAACAGTCACCACCTAGAACTGGGTATTCTTTGTACATTGTCAGATATCTTTCAAAGT
  ATAAATTAGGAATAAAAATGTTCCACATGATATGTGTACAAATTCTTTTTAAGATTGTCATCTTGTACAG
  CAAAATATTATGTTGGTATATTACTTCCTTATTAATTTGCAAATGATTGGATTAAAAAAGCTCACTGTAT
  TCCTTACTAACCTGTTGTGTGAAACATAACATTGGCAGTCGAGGAGTCAGAACTCGGATTCCCAGCCTCA
  CTCCCTCTGTGTGTGTTGACCACGTCTCTGCTGTGAATAGGATTTCATGCAGTGGTCTGTTATGGGGCTT
  TGCAGCGTGGGGGGCTGCAGAAGATTCTCAGACATTCTTATCCTTCTGGAGTCTCAACCCATCAGGTTCA
  GACCAGTAGGAACCAGGCTGGGTCAGGCTCTTAATTTCACTATGGTGGGGGACAAAATGAACACTGGCTT
  TTGTTCACTTTGCCCCTGAGAGATCTAGTCCTGTCCTGTGATGGCTTCCACAAACCCTGTTGGAAGTTTG
  GGTTATTCCTATAACTGTATGTGTTGAGTGGCCGTGAGGCTATGGACCTAGCCTGGAATTTAGATGTCTA
  GCTATTTTTTAATCTTGTTGGTAAATGCTCAGAGGTCTGTGCTACCTCTGTGAGGTATATACTAAAATGA
  ATGTAAAATAAGGATCGTATTCCCTGCTTTAAGGAACTGGTAGTCTTGTTTGGGTGATGAGACAAAGTTC
  TTGAGACATTTAAACTGTATTTACCAGTGTGAAAAATGTCATTTATGGAGATTAATTCATTATGGAAATA
  AAACATTGCCTAAGCCCTTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002913.3
• Summary: 'This gene encodes the large subunit of replication factor C, a five subunit DNA polymerase accessory protein, which is a DNA-dependent ATPase required for eukaryotic DNA replication and repair. The large subunit acts as an activator of DNA polymerases, binds to the 3' end of primers, and promotes coordinated synthesis of both strands. It may also have a role in telomere stability. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Mar 2011]'
• Locus ID: 5981