ARID1A (NM_139135) Human 3' UTR Clone

CAT#: SC214120

3`UTR clone of AT rich interactive domain 1A (SWI-like) (ARID1A) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ARID1A
• Synonyms: B120; BAF250; BAF250a; BM029; C1orf4; CSS2; ELD; hELD; hOSA1; MRD14; OSA1; P270; SMARCF1
• ACCN: NM_139135
• Insert Size: 1395
• Sequence Data:
  Insert Sequence

  >SC214120 3'UTR clone of NM_139135
  The sequence shown below is from the reference sequence of NM_139135. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CACAAGTCATTTGTGATGTACTGTTTTTGATTGGCCAGTCATGACAGCCGTGGGACACCTCCCCCCCCCG
  TGTGTGTGTGCGTGTGTGGAGAACTTAGAAACTGACTGTTGCCCTTTATTTATGCAAAACCACCTCAGAA
  TCCAGTTTACCCTGTGCTGTCCAGCTTCTCCCTTGGGAAAAAGTCTCTCCTGTTTCTCTCTCCTCCTTCC
  ACCTCCCCTCCCTCCATCACCTCACGCCTTTCTGTTCCTTGTCCTCACCTTACTCCCCTCAGGACCCTAC
  CCCACCCTCTTTGAAAAGACAAAGCTCTGCCTACATAGAAGACTTTTTTTATTTTAACCAAAGTTACTGT
  TGTTTACAGTGAGTTTGGGGAAAAAAAATAAAATAAAAATGGCTTTCCCAGTCCTTGCATCAACGGGATG
  CCACATTTCATAACTGTTTTTAATGGTAAAAAAAAAAAAAAAAAATACAAAAAAAAATTCTGAAGGACAA
  AAAAGGTGACTGCTGAACTGTGTGTGGTTTATTGTTGTACATTCACAATCTTGCAGGAGCCAAGAAGTTC
  GCAGTTGTGAACAGACCCTGTTCACTGGAGAGGCCTGTGCAGTAGAGTGTAGACCCTTTCATGTACTGTA
  CTGTACACCTGATACTGTAAACATACTGTAATAATAATGTCTCACATGGAAACAGAAAACGCTGGGTCAG
  CAGCAAGCTGTAGTTTTTAAAAATGTTTTTAGTTAAACGTTGAGGAGAAAAAAAAAAAAGGCTTTTCCCC
  CAAAGTATCATGTGTGAACCTACAACACCCTGACCTCTTTCTCTCCTCCTTGATTGTATGAATAACCCTG
  AGATCACCTCTTAGAACTGGTTTTAACCTTTAGCTGCAGCGGCTACGCTGCCACGTGTGTATATATATGA
  CGTTGTACATTGCACATACCCTTGGATCCCCACAGTTTGGTCCTCCTCCCAGCTACCCCTTTATAGTATG
  ACGAGTTAACAAGTTGGTGACCTGCACAAAGCGAGACACAGCTATTTAATCTCTTGCCAGATATCGCCCC
  TCTTGGTGCGATGCTGTACAGGTCTCTGTAAAAAGTCCTTGCTGTCTCAGCAGCCAATCAACTTATAGTT
  TATTTTTTTCTGGGTTTTTGTTTTGTTTTGTTTTCTTTCTAATCGAGGTGTGAAAAAGTTCTAGGTTCAG
  TTGAAGTTCTGATGAAGAAACACAATTGAGATTTTTTCAGTGATAAAATCTGCATATTTGTATTTCAACA
  ATGTAGCTAAAACTTGATGTAAATTCCTCCTTTTTTTCCTTTTTTGGCTTAATGAATATCATTTATTCAG
  TATGAAATCTTTATACTATATGTTCCACGTGTTAAGAATAAATGTACATTAAATCTTGGTAAGAC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_139135.2
• Summary: This gene encodes a member of the SWI/SNF family, whose members have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. It possesses at least two conserved domains that could be important for its function. First, it has a DNA-binding domain that can specifically bind an AT-rich DNA sequence known to be recognized by a SNF/SWI complex at the beta-globin locus. Second, the C-terminus of the protein can stimulate glucocorticoid receptor-dependent transcriptional activation. It is thought that the protein encoded by this gene confers specificity to the SNF/SWI complex and may recruit the complex to its targets through either protein-DNA or protein-protein interactions. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
• Locus ID: 8289