SKP1 (NM_170679) Human 3' UTR Clone

CAT#: SC214133

3`UTR clone of S-phase kinase-associated protein 1 (SKP1) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: SKP1
• Synonyms: EMC19; OCP-II; OCP2; p19A; SKP1A; TCEB1L
• ACCN: NM_170679
• Insert Size: 1373 bp
• Sequence Data:
  Insert Sequence

  >SC214133 3'UTR clone of NM_170679
  The sequence shown below is from the reference sequence of NM_170679. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  ACGCAAAGAGAACCAGTGGTGTGAAGAGAAGTGAAATGTTGTGCCTGACACTGTAACACTGTAAGGATTG
  TTCCAAATACTAGTTGCACTGCTCTGTTTATAATTGTTAATATTAGACAAACAGTAGACAAATGCAGCAG
  CAAGTCAATTGTATTAGCAGAATATTGTCCTCATTGCATGTGTAGTTTGAGCACAGATCCCAAACCTTAC
  GGCCAAGTTTCTTCTAGTATGATGGAAAGTTTCTTTTTTCTTTGCTCTGAATAAAACTGAACTGTGGGTT
  CTCTATAAGTGGCATTTTGGGCTTTCCCTCTTTTTTGTAAAGCAATGTCTGCCTAGTTTATTGTCCAGTT
  AACTTTAGTGACCTTTTAAAAGTTGGCATTGTAAATAAAACAACTTGCAAAAAAGTTTTCTGGAATAGAA
  TTAACAAAATATTATCTTTATTCATGAGTTGGAAACTGGAAAAAGGCTTCTTGAAGTAAATGTTCTGAGT
  GGAGCTACTAGGATGTCTTCCAGCCTCCTGCAGTCAAGGAGTACCACTGTATTGATTAGCCTGTATGTAG
  CAGGGCTCCCTTCATTGCATCTGAGGACTTGTTTTCTTTTTCTTTATTTTTAATCCTCTTAGTTTTAAAT
  ATATTGCCTAGAGACTCAGTTACTACCCAGTTTGTGGTTTTTTGGGAGAAATGTAACTGGACAGTTAGCT
  TTTCAATTAAAAAGACACTTAACCCATGTGGGATGTCATCTTTTTATAATTAGTGTTCCCATGTGGAGAA
  AATTATTCACACTACTTGCATGTAAAGAATAATTTAACTTTTAACATTAAAATATGTGGTAAAACCCAGA
  AAGCATCCATCATGAATGCAAGATACTTTCAATAAAAAGTAAGTTATATAGTAGGTAGTTAAGTTTGCTT
  TTGTGGACTTAAATGTGTCTCTTCACTTAAATGGGTTGAATGTGTATATATTTGTTCAGCTTGAAAAGAC
  TTAGTTTATATCCTAGCTCACTGGAGGCTGCTGACATAACCATAACTTCTGTCCCTTCTAATTGTCATTT
  ATATGCCTAACTGGAGCTAGTACTTTAATTCTTAACACAAAATTACTCTGCCATTGTTTCCAGCTTCCCT
  CCTACAATAGAATGAAGTTTTTTTGATGGCTTGAGATGGCTCACAAATTTTGATTTTTTTTTCTTCCTTG
  TGCTCCCTTTTTTTCTCCTTGCTTTTCCAGTTAACATCTATATTCACATGTAATCTTGTTTTCTCTTCAC
  ATTCACTGAGTTGTTCAGGCTCAGATCATCCCTTGACAGTAGTTTGCCTTCATCTCACCTTTCATTTGTC
  CCAAATTCACCTTATTTAATAAAGTCCCATATGTTGTCTCACT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_170679.2
• Summary: 'This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteosome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7. [provided by RefSeq, Jul 2008]'
• Locus ID: 6500