Eph receptor B1 (EPHB1) (NM_004441) Human 3' UTR Clone

CAT#: SC214149

3`UTR clone of EPH receptor B1 (EPHB1) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: EPHB1
• Synonyms: ELK; EPHT2; Hek6; NET
• ACCN: NM_004441
• Insert Size: 1367 bp
• Sequence Data:
  Insert Sequence

  >SC214149 3'UTR clone of NM_004441
  The sequence shown below is from the reference sequence of NM_004441. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TCAGTCACCAACGGCAATGGCATGAGAACTCTTGTTTCTTGGGGAAGGAGAGGAGGGAAAAGGACCAGGG
  TCAAGGGGGACCAGAGGTTGACCACTGTGGAATGTACTGGAGAGACTGGCTTCTCAGCTGAGGAATGCAT
  TTCCATCAGTGAAGAATCAACCGGACCTGTTGCTAGCAGGCAATCTCCATTTCTCAGTGACAGAAGCATG
  TTTGAGATGCCGTGGGAAACCAAATATATAATAATAAAAATATAAAAAGGTGATGTTCAACAGAAGTGAA
  GACAAAACAATATGCATCAGGAGAACAAGAGTAAACCCAGCTCCCACTCTCAGTGGGCTGCAGTTGCCCA
  ACCACAGGAAGAAAGGGAAGGAGGTAGAGGGAAGAAACAGAAGCAGTGTTCCATTTTCTTCCTCACCAAT
  GACATTCTTTTCTTTTCTCCTTTCGTACTCCTCCCTGAGAGTCCCCTCCCTTCTCCCACACTCGTTTCCC
  TTTGCTCATGACTCCTGTAGGGAAGTTTCTTCAAACAAAACCCAGCTCCTGAGTCTCCAGATGTTGTTCT
  GTCAGTTGCCAAAGGACTTTGCTGACCACTGCATGGGGATCCAACCAATTCAATTAATGTCTTCATATTG
  AAGAAGAGATGTACCTTCAATTGAAAACCTCGTTTTTCTTTTGTTTGCATTTTCTGCAAAAAGGAAAAAG
  AAACCACAAATTGGGGAAAAAAAAAGAAGAAAAACCTGTTTCCGTGTGCAAAAGCACACATATGTATGTC
  TGTGTTATAAAATGACTGTGCTTGTTCGTAACAGATGCAAACAAGAAAGAAGAACTGGGAAGTCTTTGTC
  CCTAGGAAATCCAAAGGGGCTGGAATATGGTGTTGGTTTGGCTTTCTGGTTGGCCCAATCGGCCTATTGG
  CTCAATGGGAAGAGAGGAGAGGGAGAAAAATAAAATGAAAGGAAAAAAAAAAGTTTGCAAATTCAGACAG
  GAAACAGGTGAGTGGTTTGAATTGGATGCAGTGTGGGCCATCCTGGAATGATACTGACTGATTAATTATT
  CCTGATAACATCTCAAGAAAAGGAGAAGGAAAGTGTTTCTGGAGAATGTTCTTTCACATCACTGGAATCT
  GCAATTCAAGAAGTGACAAGGGAGAATTCTTGCTTTACCTATGGACTGGCTTAAGCCGTGTGGCATCCGA
  GGAATGTTTCAAATGTGTCTGTGTTTCTCTTTACATTCCTTGTTGTACCTCATTGTTCAATTCACTTTTG
  TAAATTCCACCTAACATTTAATTATTTTAAATTTCTCCTTTTACCTTAATCTCCTTGCTAATTTTATCTG
  TCTAATTAAAAAGAGCAGAAGCATGTCTGGGTTTACG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_004441.4
• Summary: 'Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]'
• Locus ID: 2047