G0 Protein alpha (GNAO1) (NM_020988) Human 3' UTR Clone

CAT#: SC214188

3`UTR clone of guanine nucleotide binding protein (G protein) alpha activating activity polypeptide O (GNAO1) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: GNAO1
• Synonyms: EIEE17; G-ALPHA-o; GNAO; HLA-DQB1; NEDIM
• ACCN: NM_020988
• Insert Size: 1386 bp
• Sequence Data:
  Insert Sequence

  >SC214188 3'UTR clone of NM_020988
  The sequence shown below is from the reference sequence of NM_020988. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATCATTGCCAACAACCTCCGGGGCTGCGGCTTGTACTGACCTCTTGTCCTGTATAGCAACCTATTTGAC
  TGCTTCACGGACTCTTTGCTGTTGACGTTGATCTCCTGGTAGCATGACCTTTTGGCCTTTGTAAGACACA
  CAGCCTTTCTGTATCAAGCCCCTGTCTAACCTACGACCCCAGAGTGACTGACGGCTGTGTATTTCTGTAG
  AATGCTGTAGAATCCGGTTTTAGTTGAGTCTTCACATTTAGAATTTGAAAGGAAAAACAGAACATTTCTC
  ATGTGCTTTGTAGCTTAAAAAAAGGAAAACTCACCATTTCATCCATATTTCTTTTTCTTGCAAAACAAAC
  ATACCCATCTGCACCGACGCCTGCCCCCGTCCCACCTCGCGGCGCAGCCCCTGGAGCTCCTGCTGGGGGG
  CAGGGCCCATCTGCCGCCTGGGGCTCCGGTGCGGTGCACTGGTCCGAGGAGTGTGCTCAGGCCGGGCTTT
  CCAGAAGGCCACAGGCCACTGCAAACCCTGCTGCCTGCCGGCCGCCCAGACACCACAGACGCCACCTGGC
  GGCCCTAAGCCACTCCCGCCACACGCTCACTCCAGGTTCGTATAGAAAAAGCACAGCTCTCACTGGCCAG
  TAGCTGCCAAAAAGAACACCCATCGTATAAGGAAATCCAGCCCCATAGAAAACAATCTGCCCACCGACCC
  CCTTTCTAAGGATAGCCTTTGTAGGGGTTTTTTGGTTCCTTTTTTTTTTTTTTCAGTTTTTGTTTTTACT
  TTTGTCCCGCCGAGTACTATGTATTACCAGCCCCCCACCCCACAGATCAGTTTTCCTAGTCTTTGGGAAA
  CGGGTTGTGTTTTTTTTCCTTTGCCGTGTTGGATTTCCGGTCCCGGCTGACACCTCCCTTCGTGTGTGCG
  CCCCTCCCCACCCCTGCCTTTGTTTCGGTGCCCTGTGTCCGCTCTCTCATGTGGGGATGTTTGTGCTGCA
  GACAAAAAGAACAAAAAAAAATTTTTAAATACCACAAGATGGAAAAAAAAAACAAAAAAATTTAAAAAGA
  TGGAATGTAGTGTTTACATTAAAGCCGCCGTTTTCATGACCAATCCTATGTCATTTCTACTTTGACTAAT
  ACCCAAACCCTAAACCTCTTCACGGTTTCACTTTTAAGACTTAATCTTTGCTGAAGAAGACCAGTCACAG
  CCATTTCAAATAAAGAGGAGGCAAAAAGACAAAACACACAAAAAATTAAAAAAAAAAACTTAAAAAAAAA
  AGGAAAAAAGAAAAAAAAGCCCACGGGTCTTTGTAAGCCTTTCTATTCCCAATCGGTGAGGTTTCTGTGA
  TATCTTACACACTGCCAGTCTACTTCTCTGACTAATGGAAAATTCCTGTGTAGCTC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_020988.2
• Summary: 'The protein encoded by this gene represents the alpha subunit of the Go heterotrimeric G-protein signal-transducing complex. Defects in this gene are a cause of early-onset epileptic encephalopathy. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
• Locus ID: 2775