Phospholamban (PLN) (NM_002667) Human 3' UTR Clone

CAT#: SC214206

3`UTR clone of phospholamban (PLN) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PLN
• Synonyms: CMD1P; CMH18; PLB
• ACCN: NM_002667
• Insert Size: 1365 bp
• Sequence Data:
  Insert Sequence

  >SC214206 3'UTR clone of NM_002667
  The sequence shown below is from the reference sequence of NM_002667. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTATCATCGTGATGCTTCTCTGAAGTTCTGCTACAACCTCTAGATCTGCAGCTTGCCACATCAGCTTAAA
  ATCTGTCATCCCATGCAGACAGGAAAACAATATTGTATAACAGACCACTTCCTGAGTAGAAGAGTTTCTT
  TGTGAAAAGGTCAAGATTAAGACTAAAACTTATTGTTACCATATGTATTCATCTGTTGGATCTTGTAAAC
  ATGAAAAGGGCTTTATTTTCAAAAATTAACTTCAAAATAAGTGTATAAAATGCAACTGTTGATTTCCTCA
  ACATGGCTCACAAATTTCTATCCCAAATCTTTTCTGAAGATGAAGAGTTTAGTTTTAAAACTGCACTGCC
  AACAAGTTCACTTCATATATAAAGCATTATTTTTACTCTTTTGAGGTGAATATAATTTATATTACAATGT
  AAAAGCTTCTTTAATACTAAGTATTTTTCAGGTCTTCACCAAGTATCAAAGTAATAACACAAATGAAGTG
  TCATTATTCAAAATAGTCCACTGACTCCTCACATCTGTTATCTTATTATAAAGAACTATTTGTAGTAACT
  ATCAGAATCTACATTCTAAAACAGAAATTGTATTTTTTCTATGCCACATTAACATCTTTTAAAGTTGATG
  AGAATCAAGTATGGAAAAGTAAGGCCATACTCTTACATAATAAAATTCCTTTTAAGTAATTTTTTCAAAG
  AATCACAGAATTCTAGTACATGTAGGTAAATCATAAATCTGTTCTAAGACATATGATCAACAGATGAGAA
  CTGGTGGTTAATATGTGACAGTGAGATTAGTCATATCACTAATATACTAACAACAGAATCTAATCTTCAT
  TTAAGGCACTGTAGTGAATTATCTGAGCTAGAGTTACCTAGCTTACCATACTATATCTTTGGAATCATGA
  AACCTTAAGACTTCAGAATGATTTTGCAGGTTGTCTTCCATTCCAGCCTAACATCCAATGCAGGCAAGGA
  AAATAAAAGATTTCCAGTGACAGAAAAATATATTATCTCAAGTATTTTTTAAAAATATATGAATTCTCTC
  TCCAAATATTAACTAATTATTAGATTATATTTTGAAATGAACTTGTTGGCCCATCTATTACATCTACAGC
  TGACCCTTGAACATGGGGGTTAGGGGAGCTGACAATTCGTGGGTCCGCAAAATCTTAACTACCTAATAGC
  CTACTATTGACCATAAACCTTACTGATAACATAAACAGTAAATTAACACATATTTTGCGTGTTATATGTA
  TTATACACTATATTCCTACAATAAAGTAAGCTAGAGAAAATGTTATTTAGAAAATCATAAGAAAGAGAAA
  ATATATTTACTATTCATTAAATGGAAGTGGGTCAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_002667.3
• Summary: 'The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure, and also familial hypertrophic cardiomyopathy. [provided by RefSeq, Apr 2016]'
• Locus ID: 5350