Caspase-7 (CASP7) (NM_033338) Human 3' UTR Clone

CAT#: SC214266

3`UTR clone of caspase 7 apoptosis-related cysteine peptidase (CASP7) transcript variant delta for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CASP7
• Synonyms: CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3
• ACCN: NM_033338
• Insert Size: 1395 bp
• Sequence Data:
  Insert Sequence

  >SC214266 3'UTR clone of NM_033338
  The sequence shown below is from the reference sequence of NM_033338. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  TGCTCACCAAGGAACTCTACTTCAGTCAATAGCCATATCAGGGGTACATTCTAGCTGAGAAGCAATGGGT
  CACTCATTAATGAATCACATTTTTTTATGCTCTTGAAATATTCAGAAATTCTCCAGGATTTTAATTTCAG
  GAAAATGTATTGATTCAACAGGGAAGAAACTTTCTGGTGCTGTCTTTTGTTCTCTGAATTTTCAGAGACT
  TTTTTTATAATGTTATTCATTTGGTGACTGTGTAACTTTCTCTTAAGATTAATTTTCTCTTTGTATGTCT
  GTTACCTTGTTAATAGACTTAATACATGCAACAGAAGTGACTTCTGGAGAAAGCTCATGGCTGTGTCCAC
  TGCAATTGGTGGTAACAGTGGTAGAGTCATGTTTGCACTTGGCAAAAAGAATCCCAATGTTTGACAAAAC
  ACAGCCAAGGGGATATTTACTGCTCTTTATTGCAGAATGTGGGTATTGAGTGTGATTTGAATGATTTTTC
  ATTGGCTTAGGGCAGATTTTCATGCAAAAGTTCTCATATGAGTTAGAGGAGAAAAAGCTTAATGATTCTG
  ATATGTATCCATCAGGATCCAGTCTGGAAAACAGAAACCATTCTAGGTGTTTCAACAGAGGGAGTTTAAT
  ACAGGAAATTGACTTACATAGATGATAAAAGAGAAGCCAAACAGCAAGAAGCTGTTACCACACCCAGGGC
  TATGAGGATAATGGGAAGAGGTTTGGTTTCCTGTGTCCAGTAGTGGGATCATCCAGAGGAGCTGGAACCA
  TGGTGGGGGCTGCCTAGTGGGAGTTAGGACCACCAATGGATTGTGGAAAATGGAGCCATGACAAGAACAA
  AGCCACTGACTGAGATGGAGTGAGCTGAGACAGATAAGAGAATACCTTGGTCTCACCTATCCTGCCCTCA
  CATCTTCCACCAGCACCTTACTGCCCAGGCCTATCTGGAAGCCACCTCACCAAGGACCTTGGAAGAGCAA
  GGGACAGTGAGGCAGGAGAAGAACAAGAAATGGATGTAAGCCTGGCCCATAATGTGAACATAAGTAATCA
  CTAATGCTCAACAATTTATCCATTCAATCATTTATTCATTGGGTTGTCAGATAGTCTATGTATGTGTAAA
  ACAATCTGTTTTGGCTTTATGTGCAAAATCTGTTATAGCTTTAAAATATATCTGGAACTTTTTAGATTAT
  TCCAAGCCTTATTTTGAGTAAATATTTGTTACTTTTAGTTCTATAAGTGAGGAAGAGTTTATGGCAAAGA
  TTTTTGGCACTTTGTTTTCAAGATGGTGTTATCTTTTGAATTCTTGATAAATGACTGTTTTTTTCTGCCT
  AATAGTAACTGGTTAAAAAACAAATGTTCATATTTATTGATTAAAAATGTGGTTGCTTAATTCCT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_033338.4
• Summary: 'This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. The precursor of the encoded protein is cleaved by caspase 3 and 10, is activated upon cell death stimuli and induces apoptosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]'
• Locus ID: 840