ATP6V0E2 (NM_145230) Human 3' UTR Clone

CAT#: SC214283

3`UTR clone of ATPase H+ transporting V0 subunit e2 (ATP6V0E2) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ATP6V0E2
• Synonyms: ATP6V0E2L; C7orf32
• ACCN: NM_145230
• Insert Size: 1393
• Sequence Data:
  Insert Sequence

  >SC214283 3'UTR clone of NM_145230
  The sequence shown below is from the reference sequence of NM_145230. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GAATGAGACCATCTGGTACGTGCGCTTCCTGTGGGAGTGACCCGCCGCCCCCGACCCAGGTGCCCAGCTC
  TCGGAATGACTGTGGCTCCACTGTCCCTGACAACCCCTTCGTCCGGACCCTCCCCCACACAACTATGTCT
  GGTCACCAGCTCCCTCCTGCTGGCACCCAGAGACCCGGACCCGCAGGGCCTGCCTGGTTCCTGGAAGTCT
  TCCCAGTCTTCCCAGCCAGCCCGGGCCCTGGGGAGCCCTGGGCACAGCAGCGGCCGAGGGGATGTCCTGC
  TCCAATACCCGCACTGCTCTGGAGTTTGCCCTCTTTCCCAAGGAGATGCTGCTGGGGAGCTGGTATGGGT
  GGGGTCTTTCCCTTTACAGACGGGGCAGATGCCAGGACTCAGCCCATCCTGAGGAGGACACGTGTCCTCA
  TGGAGAGGGTGCTCCGGCCCAGGCGGGGGAGTCAGTGCCCAGTCAGCAGCTCTGCCACCATCCTGCTGGG
  AACTGGGGGGGCCTCTATTGGGTTATAGGCAAGGCCTTTTCTCTGGCATGGAATTGTTAATTTTCTGACA
  CGTCTAGATGTGAAATTTCTGAAAATGTTGAAGCAGAGAAACATTCACACACAAAAAGCAACATAGTCAT
  GTGGGTCCAGATGGCCTCAGTCCTAGATGTTGGCACCCTTTGCTGTGTCTCCTCAGAGTATCCTGTTCCG
  CCTCCTGCCACCTGGACCTCCCTCAGTGGATGTCTTCCCTCCCCCGACCCCAGCCTGTCAGTCCGAGCAC
  AGTGCAGGTTTGGCTCTGACTTGGGCTTTTGGCTGCAGTGGGGGTGGATTTCAGAGCCTCTCATGGCAGC
  ATCTAAGTGACCAGAGCTGGGATGAGAGAGGGGAAGGGGCAATGTGAGTGGCGCTATGGGACGGGCCAGC
  CCTGCTCCTGAGCCAGCCCCGCCCTCTGCCCCCTGGCCCTGGGCTCTGTGCTAGGGATGGTGAAGAATGG
  GGGCGTGCCAGCCTGGCAGGAGTGGGAAGCAACACGCAGGGGTCCCGGACCTCTCCAGCCTTGCCCTCAC
  GCTTACCCGAGCTCCCAGTGTGGTTAGCACAGAGCTCACCCACCTTGCCTGGCTCCCAGCTGGGGCCTGT
  CCTCACTGGTGCTCCAGGGGAAGAAACGACAGCCTCACTTCTGTATGGACTGCTGATGTGGCCTGCCATC
  CTGTTCAGCGGGCATTGTCTTTGGAGCAGCAGGAGAATAGGATGCCTCTCACTCACATGCCAGTTCCTGG
  CTGGCCAGCTGCTCAGGGCTCAGGCTGGGGCCTCCCATTGACATCCTCCCCCTACACTCCCTCTCTGAGC
  CTCCGTCGCCCCTCCTGTTGGGTAAGGGTGTTGAGTGTGACTTGTGCTGAAAACCTGGTTCAT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_145230.2
• Summary: Multisubunit vacuolar-type proton pumps, or H(+)-ATPases, acidify various intracellular compartments, such as vacuoles, clathrin-coated and synaptic vesicles, endosomes, lysosomes, and chromaffin granules. H(+)-ATPases are also found in plasma membranes of specialized cells, where they play roles in urinary acidification, bone resorption, and sperm maturation. Multiple subunits form H(+)-ATPases, with proteins of the V1 class hydrolyzing ATP for energy to transport H+, and proteins of the V0 class forming an integral membrane domain through which H+ is transported. ATP6V0E2 encodes an isoform of the H(+)-ATPase V0 e subunit, an essential proton pump component (Blake-Palmer et al., 2007 [PubMed 17350184]). [supplied by OMIM, Mar 2008]
• Locus ID: 155066