Factor IX (F9) (NM_000133) Human 3' UTR Clone

CAT#: SC214304

3`UTR clone of coagulation factor IX (F9) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: F9
• Synonyms: F9 p22; FIX; HEMB; P19; PTC; THPH8
• ACCN: NM_000133
• Insert Size: 1402 bp
• Sequence Data:
  Insert Sequence

  >SC214304 3'UTR clone of NM_000133
  The sequence shown below is from the reference sequence of NM_000133. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CGGTATGTCAACTGGATTAAGGAAAAAACAAAGCTCACTTAATGAAAGATGGATTTCCAAGGTTAATTCA
  TTGGAATTGAAAATTAACAGGGCCTCTCACTAACTAATCACTTTCCCATCTTTTGTTAGATTTGAATATA
  TACATTCTATGATCATTGCTTTTTCTCTTTACAGGGGAGAATTTCATATTTTACCTGAGCAAATTGATTA
  GAAAATGGAACCACTAGAGGAATATAATGTGTTAGGAAATTACAGTCATTTCTAAGGGCCCAGCCCTTGA
  CAAAATTGTGAAGTTAAATTCTCCACTCTGTCCATCAGATACTATGGTTCTCCACTATGGCAACTAACTC
  ACTCAATTTTCCCTCCTTAGCAGCATTCCATCTTCCCGATCTTCTTTGCTTCTCCAACCAAAACATCAAT
  GTTTATTAGTTCTGTATACAGTACAGGATCTTTGGTCTACTCTATCACAAGGCCAGTACCACACTCATGA
  AGAAAGAACACAGGAGTAGCTGAGAGGCTAAAACTCATCAAAAACACTACTCCTTTTCCTCTACCCTATT
  CCTCAATCTTTTACCTTTTCCAAATCCCAATCCCCAAATCAGTTTTTCTCTTTCTTACTCCCTCTCTCCC
  TTTTACCCTCCATGGTCGTTAAAGGAGAGATGGGGAGCATCATTCTGTTATACTTCTGTACACAGTTATA
  CATGTCTATCAAACCCAGACTTGCTTCCGTAGTGGAGACTTGCTTTTCAGAACATAGGGATGAAGTAAGG
  TGCCTGAAAAGTTTGGGGGAAAAGTTTCTTTCAGAGAGTTAAGTTATTTTATATATATAATATATATATA
  AAATATATAATATACAATATAAATATATAGTGTGTGTGTATGCGTGTGTGTAGACACACACGCATACACA
  CATATAATGGAAGCAATAAGCCATTCTAAGAGCTTGTATGGTTATGGAGGTCTGACTAGGCATGATTTCA
  CGAAGGCAAGATTGGCATATCATTGTAACTAAAAAAGCTGACATTGACCCAGACATATTGTACTCTTTCT
  AAAAATAATAATAATAATGCTAACAGAAAGAAGAGAACCGTTCGTTTGCAATCTACAGCTAGTAGAGACT
  TTGAGGAAGAATTCAACAGTGTGTCTTCAGCAGTGTTCAGAGCCAAGCAAGAAGTTGAAGTTGCCTAGAC
  CAGAGGACATAAGTATCATGTCTCCTTTAACTAGCATACCCCGAAGTGGAGAAGGGTGCAGCAGGCTCAA
  AGGCATAAGTCATTCCAATCAGCCAACTAAGTTGTCCTTTTCTGGTTTCGTGTTCACCATGGAACATTTT
  GATTATAGTTAATCCTTCTATCTTGAATCTTCTAGAGAGTTGCTGACCAACTGACGTATGTTTCCCTTTG
  TG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000133.3
• Summary: 'This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]'
• Locus ID: 2158