Factor IX (F9) (NM_000133) Human 3' UTR Clone
CAT#: SC214304
3`UTR clone of coagulation factor IX (F9) for miRNA target validation
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Specifications
Product Data | |
Vector | pMirTarget |
Species | Human |
Transfection Reporter | RFP |
Assay Reporter | Luciferase |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Symbol | F9 |
Synonyms | F9 p22; FIX; HEMB; P19; PTC; THPH8 |
ACCN | NM_000133 |
Insert Size | 1402 bp |
Sequence Data |
>SC214304 3'UTR clone of NM_000133
The sequence shown below is from the reference sequence of NM_000133. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon CAATTGGCAGAGCTCAGAATTCAAGCGATCGC CGGTATGTCAACTGGATTAAGGAAAAAACAAAGCTCACTTAATGAAAGATGGATTTCCAAGGTTAATTCA TTGGAATTGAAAATTAACAGGGCCTCTCACTAACTAATCACTTTCCCATCTTTTGTTAGATTTGAATATA TACATTCTATGATCATTGCTTTTTCTCTTTACAGGGGAGAATTTCATATTTTACCTGAGCAAATTGATTA GAAAATGGAACCACTAGAGGAATATAATGTGTTAGGAAATTACAGTCATTTCTAAGGGCCCAGCCCTTGA CAAAATTGTGAAGTTAAATTCTCCACTCTGTCCATCAGATACTATGGTTCTCCACTATGGCAACTAACTC ACTCAATTTTCCCTCCTTAGCAGCATTCCATCTTCCCGATCTTCTTTGCTTCTCCAACCAAAACATCAAT GTTTATTAGTTCTGTATACAGTACAGGATCTTTGGTCTACTCTATCACAAGGCCAGTACCACACTCATGA AGAAAGAACACAGGAGTAGCTGAGAGGCTAAAACTCATCAAAAACACTACTCCTTTTCCTCTACCCTATT CCTCAATCTTTTACCTTTTCCAAATCCCAATCCCCAAATCAGTTTTTCTCTTTCTTACTCCCTCTCTCCC TTTTACCCTCCATGGTCGTTAAAGGAGAGATGGGGAGCATCATTCTGTTATACTTCTGTACACAGTTATA CATGTCTATCAAACCCAGACTTGCTTCCGTAGTGGAGACTTGCTTTTCAGAACATAGGGATGAAGTAAGG TGCCTGAAAAGTTTGGGGGAAAAGTTTCTTTCAGAGAGTTAAGTTATTTTATATATATAATATATATATA AAATATATAATATACAATATAAATATATAGTGTGTGTGTATGCGTGTGTGTAGACACACACGCATACACA CATATAATGGAAGCAATAAGCCATTCTAAGAGCTTGTATGGTTATGGAGGTCTGACTAGGCATGATTTCA CGAAGGCAAGATTGGCATATCATTGTAACTAAAAAAGCTGACATTGACCCAGACATATTGTACTCTTTCT AAAAATAATAATAATAATGCTAACAGAAAGAAGAGAACCGTTCGTTTGCAATCTACAGCTAGTAGAGACT TTGAGGAAGAATTCAACAGTGTGTCTTCAGCAGTGTTCAGAGCCAAGCAAGAAGTTGAAGTTGCCTAGAC CAGAGGACATAAGTATCATGTCTCCTTTAACTAGCATACCCCGAAGTGGAGAAGGGTGCAGCAGGCTCAA AGGCATAAGTCATTCCAATCAGCCAACTAAGTTGTCCTTTTCTGGTTTCGTGTTCACCATGGAACATTTT GATTATAGTTAATCCTTCTATCTTGAATCTTCTAGAGAGTTGCTGACCAACTGACGTATGTTTCCCTTTG TG ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG |
Restriction Sites | SgfI-MluI |
OTI Disclaimer | Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs). |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. |
Reference Data | |
RefSeq | NM_000133.3 |
Summary | 'This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing. [provided by RefSeq, Sep 2015]' |
Locus ID | 2158 |
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