TAF4 (NM_003185) Human 3' UTR Clone

CAT#: SC214319

3`UTR clone of TAF4 RNA polymerase II TATA box binding protein (TBP)-associated factor 135kDa (TAF4) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: TAF4
• Synonyms: TAF2C; TAF2C1; TAF4A; TAFII130; TAFII135
• ACCN: NM_003185
• Insert Size: 1388 bp
• Sequence Data:
  Insert Sequence

  >SC214319 3'UTR clone of NM_003185
  The sequence shown below is from the reference sequence of NM_003185. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GACAAGCCATTCACTGCTGCTCTACAAAGCATTCCTTAAGTGACACAGGAGGACGCCTGGGGACTTTTTA
  TATATTTGCAGATTACGCCTTTTTGTAACGAGCAAATGGGATATTGTTTAAAAAACAGCCACCTCTTTAC
  AATGGAACAGTTTTATATTCCTGTTTCTAAATCAGCTCTTCAGTGTGAAAGAAAACACGTTTCTGTAACA
  GAGAGAACACAAAGGCCTGTGGATACTCTTAAAGGACAATTAAATCTTAACTCATCTTGATTGAGTGGCC
  TTCCTGCCAAACAAGCCATATATAAAGACTGATGGAATCGTTAGCAAATAATTAGCTGCCCTCTGTCAAC
  TCATAGCAGTTTCTGCATTATTTGTGCATTTTGGTTTAGTTCTACCTAACTTACTATGTAGGTGTATGTC
  TACAGCCGATGACCTCATTTCGTTTATTTTATTTTTGTAATAGTCAGTTGGCAAAGCAAACTGATTTTTT
  AGACTATTTATCTTCCTTCCCTTCCCCTCCCACCCCGCTCTCCTCTCTGCCCCCTGCCCTCCCCTCCCCT
  CCCTTCCCCTCCACTCCGCTGAGAATCCTGGAGGAATACACAATTCATCGTTGCACCCCCACCTCAGAGT
  GTAATCGCATTTCTGCTTGGTAGAGGCCGAGCCCAGCAAAGGTGGCTCCTTCTGAATGTGTGGTCAGCAT
  CTGTACAAATGCATTTTATTTGCTATAGTTTGTAAAGCTGTAAAGTTAAAAGAGATGAAAACCTTTTCAG
  CATAAATATATTTTACTTGCACTGTGTTTTTTAGCTAAAAGTGAAAACCTAGATTAAATAAAATCAAAGT
  TGAGAAGAATCATCAAAAGACTGTTTCTCGGTGTGAATCAAGTGTTGAAAAATGGTTGGTGTATTTTGTC
  AGTAATTGTACATAACTTTTGGCACATGACATAGAAATGGCTATGTAAACTATAATTATTTTGCTAAGAG
  ACTGTATGCAAGCCTTGGGCCGACTTTACAGACGTCCAGAGCAAAGCCCCTTCTTTGTACCTATTTTTTT
  ATTACAAATATACTAATTGGTTCTTTCTATTTTCAGAGGTTATTGTATGAAATTGTCTATTGATAGTACT
  TTTATGACTGTAAATACTCTGGCTTTCTCCGTGTGAATTCTCACATTAGACTTTAATTCGAGCGCGTGTG
  AACTGAACGCTGATCAGTATTTTTTATCAACACCTGAGAACTGTTACACCTTTTATTTTGTCTTTTAGGA
  AATCCCTGTCTTTCCATTTTTTCATGTAAATTTTGCACAGTTACTTGTTCATATGTAAATATTTTACTTT
  CAGAAATGAAGTTTTTAATTGCTATTGTTTTATATAGGATTGAAAGAAAATTAACTCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003185.3
• Summary: 'Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides. The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals. TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs. TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation. This gene encodes one of the larger subunits of TFIID that has been shown to potentiate transcriptional activation by retinoic acid, thyroid hormone and vitamin D3 receptors. In addition, this subunit interacts with the transcription factor CREB, which has a glutamine-rich activation domain, and binds to other proteins containing glutamine-rich regions. Aberrant binding to this subunit by proteins with expanded polyglutamine regions has been suggested as one of the pathogenetic mechanisms underlying a group of neurodegenerative disorders referred to as polyglutamine diseases. [provided by RefSeq, Jul 2008]'
• Locus ID: 6874