UDP glucose dehydrogenase (UGDH) (NM_003359) Human 3' UTR Clone

CAT#: SC214428

3`UTR clone of UDP-glucose dehydrogenase (UGDH) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: UGDH
• Synonyms: GDH; UDP-GlcDH; UDPGDH; UGD
• ACCN: NM_003359
• Insert Size: 1425 bp
• Sequence Data:
  Insert Sequence

  >SC214428 3'UTR clone of NM_003359
  The sequence shown below is from the reference sequence of NM_003359. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GTCTTCAAGATCCACCTAACAAGAAACCTAAAGTGTAGAGATTGCCATTTTTATTTGTGATTTTTTTTTT
  TTTTTTTTGGTACTTCAGGATAGCAAATATCTATCTGCTATTAAATGGTAAATGAACCAAGTGTTTTTTT
  TTGTTTTTTTTTTGAGACAGAGTCTCACTGTTGCCCAGGCTGGAGTGCAGTGGTGCAATCTCGGCTCACT
  GCAAGCTCTGCTTCCCAGGTTCACGCCATTCTCCTGGCTCAGCCTCCCAAGTAGCTGGGACTACAGGCAC
  CCGCCACAGTGCCTGGCTAATTTTTTGTATTTTTAGTAGAGACAGGGTTTCACCATGTGAGCCAGGATGG
  TCTCAATCTCCTGACCTTGTGAACCACCCGTCTCGGCCTCCCAAAGTGCTGGGATTACAGGTGTGAGCCA
  CCACGCCTGGCCCATGAACCAAGTGTTTTTAAGGAAACAAAACTATTTTTTTAATCATCAGATTTATACT
  AGCTATATGGATATTAGCATATCTGGTAATTATGAATCTAGAATTTTTTTACATATTTTTATAATACTGT
  TAGCTCAGTTATTGGATGAGTGAAAGATAATCATGTTGGTTTTAATAGTGTCAATTTTTGTAAAATAAAA
  ATTAAACTTCAAACTCTTTACTTTATAAATTGTCCATAGGCCACACTTTAATATCACATTATAAAGGGAA
  GGACAGTCTTCATTCCTCCTGGTTATTGGTTTGTTTGTCATTAAAGATATATTTTGAATCCATGAAATTG
  CTATGCTAAACAGCCTTTACATGTATGGTCTGGTTAAAGTTCCTTTGTTCCTTTTGTTTTAATAAAATGT
  GTCACTGATTTTTTAGCTCAAAATCATCACTGTTAATTTCCAGTCACCCCAAATATGGTTAAAAGATTTT
  TTTTTTAATCATGAAGAGAAAATTAGTAGCATTTCTTTCTCTCCCCATTATTTATTGGTTTTCCTCACTA
  ATCTTTTTTTTTTTAGTCCAAAAGCCAAAAATATTTATCTTGGTTTTACATTTTAATTTCCATTCTTAAT
  TGTAATTTTTTTCTTTAAATAAGGAAACCAATATAATCTCATGTATAAAAACTTAAATATTTTACAAGTT
  ACATATAGCATCATTCTAAAATAAGAATTTTTTTTGTTTTCTGTCTGCTTTTTTCTTATGTCTCTTGTTG
  AGTTTTATATTTTCAGTGGTTATTTTTGCTTGTGTTAGATCATTATTAAAATATATCCAATGTCCCTTTG
  ATACTTGTGCTCTGCTGAGAATGTACAGTTTGCATTAAACATCCCAGGTCTCATCCTTCAGGAATTTTGC
  AGTTCAATGAGAAGAGGGAGACAAATATAAAGATGAGGACAGAAGCATCTCTACAGATGAAAATTACATA
  AATAAAACATTCTCCATCAACAACT
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_003359.2
• Summary: 'The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]'
• Locus ID: 7358