EVI1 (MECOM) (NM_005241) Human 3' UTR Clone

CAT#: SC214443

3`UTR clone of MDS1 and EVI1 complex locus (MECOM) transcript variant 2 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: MECOM
• Synonyms: AML1-EVI-1; EVI1; KMT8E; MDS1; MDS1-EVI1; PRDM3; RUSAT2
• ACCN: NM_005241
• Insert Size: 1408 bp
• Sequence Data:
  Insert Sequence

  >SC214443 3'UTR clone of NM_005241
  The sequence shown below is from the reference sequence of NM_005241. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GCTATCCAGTCCATAAGCCACGTATGACGTTATCAAGGTTGACCAGAGTGGGACCAAGTCCAACAGTAGC
  ATGGCTCTTTCATATAGGACTATTTACAAGACTGCTGAGCAGAATGCCTTATAAACCTGCAGGGTCACTC
  ATCTAAAGTCTAGTGACCTTAAACTGAATGATTTAAAAAAGAAAAGAAAGAAAAAAGAAACTATTTATTC
  TCGATATTTTGTTTTGCACAGCAAAGGCAGCTGCTGACTTCTGGAAGATCAATCAATGCGACTTAAAGTG
  ATTCAGTGAAAACAAAAAACTTGGTGGGCTGAAGGCATCTTCCAGTTTACCCCACCTTAGGGTATGGGTG
  GGTGAGAAGGGCAGTTGAGATGGCAGCATTGATATGAATGAACACTCCATAGAAACTGAATTCTCTTTTG
  TACAAGATCACCTGACATGATTGGGAACAGTTGCTTTTAATTACAGATTTAATTTTTTTCTTCGTTAAAG
  TTTTATGTAATTTAACCCTTTGAAGACAGAAGTAGTTGGATGAAATGCACAGTCAATTATTATAGAAACT
  GATAACAGGGAGTACTTGTTCCCCCTTTTGCCTTCTTAAGTACATTGTTTAAAACTAGGGAAAAAGGGTA
  TGTGTATATTGTAAACTATGGATGTTAACACTCAAAGAGGTTAAGTCAGTGAAGTAACCTATTCATCACC
  AGTACCGCTGTACCACTAATAAATTGTTTGCCAAATCCTTGTAATAACATCTTAATTTTAGACAATCATG
  TCACTGTTTTTAATGTTTATTTTTTTGTGTGTGTTGCGTGTATCATGTATTTATTTGTTGGCAAACTATT
  GTTTGTTGATTAAAATAGCACTGTTCCAGTCAGCCACTACTTTATGACGTCTGAGGCACACCCCTTTCCG
  AATTTCAAGGACCAAGGTGACCCGACCTGTGTATGAGAGTGCCAAATGGTGTTTGGCTTTTCTTAACATT
  CCTTTTTGTTTGTTTGTTTTGTTTTCCTTCTTAATGAACTAAATACGAATAGATGCAACTTAGTTTTTGT
  AATACTGAAATCGATTCAATTGTATAAACGATTATAATTTCTTTCATGGAAGCATGATTCTTCTGATTAA
  AAACTGTACTCCATATTTTATGCTGGTTGTCTGCAAGCTTGTGCGATGTTATGTTCATGTTAATCCTATT
  TGTAAAATGAAGTGTTCCCAACCTTATGTTAAAAGAGAGAAGTAAATAACAGACTGTATTCAGTTATTTT
  GCCCTTTATTGAGGAACCAGATTTGTTTTCTTTTTGTTTGTAATCTCATTTTGAAATAATCAGCAAGTTG
  AGGTACTTTCTTCAAATGCTTTGTACAATATAAACTGTTATGCCTTTCAGTGCATTACTATGGGAGGAGC
  AACTAAAA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_005241.3
• Summary: 'The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis, apoptosis, development, and cell differentiation and proliferation. The encoded protein can interact with CTBP1, SMAD3, CREBBP, KAT2B, MAPK8, and MAPK9. This gene can undergo translocation with the AML1 gene, resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Mar 2011]'
• Locus ID: 2122