PANK1 (NM_148977) Human 3' UTR Clone

CAT#: SC214458

3`UTR clone of pantothenate kinase 1 (PANK1) transcript variant alpha for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: PANK1
• Synonyms: PANK
• ACCN: NM_148977
• Insert Size: 1446
• Sequence Data:
  Insert Sequence

  >SC214458 3'UTR clone of NM_148977
  The sequence shown below is from the reference sequence of NM_148977. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  GGCACTGTTGGAACTGTTCAAAATGACTGATGACAAGTAGAGACGAGCAGTGGAGGAAACAGCCTCCCAA
  AAGGACAGAGAACTAAAAAATTGCTGCTGGAGAAGGTGAAAGTCGCTTTGGGACGGAAGCCAAGCCATTA
  TGGCAGATGAACCTGCTGGATTTGTAAATAATTTAAAATCCTTCCAGATGATCTTTTACTCTTAGGTTTT
  GAGCTAATGATTCAAAACGGGGGAATATAAAAGGTTTTTTTTCTGTATACTGTATTTTTTTAAAAAAATG
  GTGCAGCGTGGCCAAACCTACCAATTGTATGCATTAACTTTGAAAAGTTGTTTGATGTTTAAGAAGGACC
  TGATATGTAAGCGCTGGTCATTTTTCTTCTGGGGTTTACTGATCAGTGTGGTGATTTTAACTTCATTTAG
  TAATTACTCTAGGAGATTTTACCTTGACTTATATTTTTCATGACGTTTCATGATTTGCTGTTGGTTTCAA
  ATGAAACTACAAATCTGGCATGTTTTACTGTGAACACTTTTGTTATTTGTTTTGTACCCTTTTTTGTCTT
  GTTTTTCTGTTTTAGTTGTCTTCTGAAAAAAGAGTCGTTCCCTCTGTTTCTGTCCTCAGATGATGTCCCT
  CCCCCTACCTGTAACCTTTCTTTGACATAATTGTTCATATCAATGAAGGTGCTGACCAGCTCAATACAAA
  GTTAAGCACAAGATCTAAAGCTCTTGAAAATGCCCGTGAAGAGAAGACTGAATGTGTTAATGAATTTAAT
  GAGTCTGGCAAAAGTTGCAAATTATATGCAAGTTTGTCCTATCGCTTATAAATGTAGTGTTTCATTGGAT
  TTATTTTATGCTAGGTTATATTAAGTTGAAATAGTCTGTGATTAAATGTCCTCATCCATGCACAGAATAT
  GAATGGCAGCAAATCTTTGTGCAAGAAATTTGAAACTTATTGGGAAAAGCCTCCCAGTAGATTAATTGTT
  CATATCAGGAGATTTAGGGTAAGTCATGGGTTGAGGTGTCAGATAGTAATATCTATTTGTTTTGTACATG
  TATATATCTAGGAACTTTGTAACAACACATCTTTAATAATGTTAAAGGTTTTTTCATTTTTAATATTTTA
  AACTAAAAACTGTACTTCAATCTCAGTTTCTAAAATTAAAAATAATTTATACTGATCTATATATTTTTTC
  TTTTTGAAAGATTTCATTAAGACTGATGGGTAACTTTCAAATGAGGGTCATGTACAAATATTGGGATGCA
  TGAGATCCCATGATCTTGTGTATTGAGCTTATTGTTGAAAGGGATTTTTGAAGGACAGAACAATTACTCC
  ATGATGAATCTTCCTTTCTCTGCCTTCTGAGCACCGTCTTTAATTTCCATATCTTCAAGTCTTGAAGAAG
  TTGATGTTAATTGAAGAATTCACTTGTCTGGTTGAAATAAAGCCTG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).

Reference Data

• RefSeq: NM_148977.1
• Summary: This gene encodes a member of the pantothenate kinase family. Pantothenate kinases are key regulatory enzymes in the biosynthesis of coenzyme A (CoA). The encoded protein catalyzes the first and rate-limiting enzymatic reaction in CoA biosynthesis and is regulated by CoA through feedback inhibition. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. This gene and an intronic miRNA on the same strand are co-regulated by the tumor suppressor p53 (see PMID 20833636). [provided by RefSeq, Apr 2011]
• Locus ID: 53354