CD8A (NM_001768) Human 3' UTR Clone

CAT#: SC214602

3`UTR clone of CD8a molecule (CD8A) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: CD8A
• Synonyms: CD8; Leu2; p32
• ACCN: NM_001768
• Insert Size: 1326 bp
• Sequence Data:
  Insert Sequence

  >SC214602 3'UTR clone of NM_001768
  The sequence shown below is from the reference sequence of NM_001768. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CAGCCTTTCGGCGAGATACGTCTAACCCTGTGCAACAGCCACTACATTACTTCAAACTGAGATCCTTCCT
  TTTGAGGGAGCAAGTCCTTCCCTTTCATTTTTTCCAGTCTTCCTCCCTGTGTATTCATTCTCATGATTAT
  TATTTTAGTGGGGGCGGGGTGGGAAAGATTACTTTTTCTTTATGTGTTTGACGGGAAACAAAACTAGGTA
  AAATCTACAGTACACCACAAGGGTCACAATACTGTTGTGCGCACATCGCGGTAGGGCGTGGAAAGGGGCA
  GGCCAGAGCTACCCGCAGAGTTCTCAGAATCATGCTGAGAGAGCTGGAGGCACCCATGCCATCTCAACCT
  CTTCCCCGCCCGTTTTACAAAGGGGGAGGCTAAAGCCCAGAGACAGCTTGATCAAAGGCACACAGCAAGT
  CAGGGTTGGAGCAGTAGCTGGAGGGACCTTGTCTCCCAGCTCAGGGCTCTTTCCTCCACACCATTCAGGT
  CTTTCTTTCCGAGGCCCCTGTCTCAGGGTGAGGTGCTTGAGTCTCCAACGGCAAGGGAACAAGTACTTCT
  TGATACCTGGGATACTGTGCCCAGAGCCTCGAGGAGGTAATGAATTAAAGAAGAGAACTGCCTTTGGCAG
  AGTTCTATAATGTAAACAATATCAGACTTTTTTTTTTTATAATCAAGCCTAAAATTGTATAGACCTAAAA
  TAAAATGAAGTGGTGAGCTTAACCCTGGAAAATGAATCCCTCTATCTCTAAAGAAAATCTCTGTGAAACC
  CCTATGTGGAGGCGGAATTGCTCTCCCAGCCCTTGCATTGCAGAGGGGCCCATGAAAGAGGACAGGCTAC
  CCCTTTACAAATAGAATTTGAGCATCAGTGAGGTTAAACTAAGGCCCTCTTGAATCTCTGAATTTGAGAT
  ACAAACATGTTCCTGGGATCACTGATGACTTTTTATACTTTGTAAAGACAATTGTTGGAGAGCCCCTCAC
  ACAGCCCTGGCCTCTGCTCAACTAGCAGATACAGGGATGAGGCAGACCTGACTCTCTTAAGGAGGCTGAG
  AGCCCAAACTGCTGTCCCAAACATGCACTTCCTTGCTTAAGGTATGGTACAAGCAATGCCTGCCCATTGG
  AGAGAAAAAACTTAAGTAGATAAGGAAATAAGAACCACTCATAATTCTTCACCTTAGGAATAATCTCCTG
  TTAATATGGTGTACATTCTTCCTGATTATTTTCTACACATACATGTAAAATATGTCTTTCTTTTTTAAAT
  AGGGTTGTACTATGCTGTTATGAGTGGCTTTAATGAATAAACATTTGTAGCATCCTCTTTAATGGG
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001768.6
• Summary: 'The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein. [provided by RefSeq, May 2020]'
• Locus ID: 925