ADH5 (NM_000671) Human 3' UTR Clone

CAT#: SC214610

3`UTR clone of alcohol dehydrogenase 5 (class III) chi polypeptide (ADH5) for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: ADH5
• Synonyms: ADH-3; ADHX; FALDH; FDH; GSH-FDH; GSNOR; HEL-S-60p
• ACCN: NM_000671
• Insert Size: 1455 bp
• Sequence Data:
  Insert Sequence

  >SC214610 3'UTR clone of NM_000671
  The sequence shown below is from the reference sequence of NM_000671. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CATTCTGGAAAGAGCATTCGAACTGTTGTAAAGATTTAATTCAAAAGAGAAAAATAATGTCCATCCTGTC
  GTGATGTGATAGGAGCAGCTTAACAGGCAGGGAGAAGCGCCTCCAACCTCACAGCCTCGTAGAGCTTCAC
  AGCTACTCCAGAAAATAGGGTTATGTGTGTCATTCATGAATCTCTATAATCAAGGACAAGGATAATTCAG
  TCATGAACCTGTTTTCTGGATGCTCCTCCACATAAATAATTGCTAGTTTATTAAGGAATATTTTAACATA
  ATAAAAGTAATTTCTACATTTGTGTGGAAATTGTCTTGTTTTATGCTGTCATCATTGTCACGGTTTGTCT
  GCCCATTATCTTCATTCTGCAAGGGAAAGGGAAAGGAAGCAGGGCAGTGGTGGGTGTCTGAAACCTCAGA
  AACATAACGTTGAACTTTTAAGGGTCTCAGTCCCCGTTGATTAAAGAACAGATCCTAGCCATCAGTGACA
  AAGTTAATCAGGACCCAAGTCTGCTTCTGTGATATTATCTTGAAGGGAGGTACTGTGCCTTGTTCATACC
  TGTACCCCAAATTCCTAGGATGGCATCTGCCCTTCAGGGGGCACTAAAATGTATTATTGAAACAGCATTC
  TGGGCTTAAATAGGTGTATGTATGTGTTGGTTGTGACTGTACTATTTCTAGTATAGTGAACTACATACTG
  AATATCCAAGTTCTCAGCACCTACTTTTGTCAAATCTTAACATTTTGCCACTTCGAGATCACATTGCCAT
  TCCTCCCCTCCAGAGGTAACAATTATCCACAATTTGATGTTTATCATTCCTGTGTTGTTGTACTTTCACT
  GTGTATAACCTAAACCATCTACTCTTTAGTACTGTTTTATATATTTTTAAGCCTCATACTTGCTCATTCT
  ACAGCTTTTTTCACTCATTATTGTATAATTATATCTGAAGCTCTCGTTCATTAATTTTAGTCCTGTGTAG
  CAGAATTCAATTACGGGAACTACCATAATTTATCTGTTCTCCAGTTGAAGGCATGAAGTTGTTGCCAGTT
  TCTGTATTATAACACTGTAGTGGAACATTCTTCTGCATTGGGCTCACTGCGTGTTACCTAAGACGTATCA
  CAGAATAAACACATTTAGCCTTATAGACATTGCCAAATTGCTCTTCAAAGTAAATGTGAGTTTTTGTGAA
  TTACATGAGTATGGAATGGTGTTTTATTATGACTTTAGTTTGCATTTTCCTCAATTCTCGTTAAATCCTT
  CATTCTAATGGACATTTTATTGTGAAGAACCTGTTCATATCCTGTGCTCAACTTTGTATTGAATTATTTT
  TCTCTGAATAATTTTTAGGAGTTCTTTTATTCTAGACATCAATCATTTGTCAGTTTTATATGTTGCAAAT
  ATCTTCTAGTCTATCTTGTGACTTTTCTTTTTACTTTATGGTATTTTGTTGAATA
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_000671.3
• Summary: 'This gene encodes a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. The encoded protein forms a homodimer. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. The human genome contains several non-transcribed pseudogenes related to this gene. [provided by RefSeq, Oct 2008]'
• Locus ID: 128