H2BC18 (NM_001024599) Human 3' UTR Clone

CAT#: SC214635

3`UTR clone of histone cluster 2 H2bf (HIST2H2BF) transcript variant 1 for miRNA target validation

Specifications

Product Data

• Vector: pMirTarget
• Species: Human
• Transfection Reporter: RFP
• Assay Reporter: Luciferase
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Symbol: H2BC18
• Synonyms: HIST2H2BF
• ACCN: NM_001024599
• Insert Size: 1374 bp
• Sequence Data:
  Insert Sequence

  >SC214635 3'UTR clone of NM_001024599
  The sequence shown below is from the reference sequence of NM_001024599. The complete sequence of this clone may contain minor differences, such as SNPs. Red=Cloning site Blue=Stop Codon
  
  
  CAATTGGCAGAGCTCAGAATTCAAGCGATCGC
  
  CCAAGTACACCAGCTCGAAGTAAGAGTGTGCAAGGGACGCAATAGATCAACCACCTAACCCCAAAGGCTC
  TTTTCAGAGCCACTTCAGTAATCGAGAAAGCAGCTGTAAACACTTGTCAGAGCGTTTGATAGCTTTTGGT
  CAGGTAGGGAGTGTTACCATGGTCACGGATGCATTCGGGTTTAGGACTAAGGAGTTTCCTGTAGTCCTGT
  AATGAGTTGGCCCTCAGTCATCCCCGTCCAGTCTTACTGAGTTGCTTGTTATCTGTGCTACGAGATTCGA
  AAGGTTTGACTCGTTCTGGGATATTTGGGAGGGATGGAGGGAAGGAGGCGGGGGGTGGTTCTACACCCCT
  TCGGCCTTTAACCTGTATCTTTAAAGTCGTTTTCCTTTTTGTTTCTGAAAGTAATGAACGAGATGAGATT
  TCCCATACAGATGAGAAACCCTCGGTCCGACCTCTTACTATTTCTTAGTGTTTTAATCTGTTTTCCTTAC
  CATATACCTACAGGCGTCTGTCACAAACTTTGTCTAATATAGCCATGTGCTTTCGTCCCTTAGCCTTTTT
  GCAAGTCCTGACTTAGCCATCCTCCCCTAACTTCCATTCCAGTGACTTCAAACAGGGAGATATATTGCCA
  CCACCCCACCCCCTGTGGAGTATTTGGCAATATCTGTAGATGTTTTGGGTTGTCAGGTGTGTGTGTGTTG
  CGGGGGAGGTGCTAGAAGTATCCAATGGGCAGAGGCCAGGAGTGCTAAGCATAAGGCACAGGACAGTCCT
  CTGGGGGGACAAAGAGCTACCTGGCCTGTAATGTCGATCTTTGGTGATTGAGAGACCCCAGCGCCCAAAG
  ACATCCCTAACCTTCAGGATTTAATCCTCTTCAGTCAAACGTTTCCTTAACCCTATCAGCCCATGTTTTT
  CTTTTCTTGGTGAAAGCTGAGCACTTCATAGGCTGTTTACAGGTCCTTCTCCACAGGAAAATACTTCCTC
  CAGGACAAGAACCCTGTCTTGGTTCCAAACTTTCCCAATTATAAGAGTCACCTTTGCGCTTGTTAAACCT
  GCTTCCAGGTGCTTCTCCTGAGGTTTCCTGATTCAGCTAGACTGGAGGTGGGACCTGACGAGGTGGTTGG
  TTTTTAGTGTTCTCAGGAGTGTGGATGTTTTAATAGGTGTTGGGTCCTCATGTTAATCGACCTGTGGGTG
  TGTCACAGTCTTTGTGTCACAGATGTCCTGAGAAAGGAAACAATTTGAGGATGAGTGGAGGGGAATTTGT
  GGTGTAGAGAAGGCCACAGTTAATATGTGGGGTGAATTTCTGAAGACCTCTCAGTTCAAAACTTGAATAA
  CTCAAGACTCATCCTGACAAAAGCCAGTGGATGTTTCTTTTGCC
  
  ACGCGTAAGCGGCCGCGGCATCTAGATTCGAAGAAAATGACCG
• Restriction Sites: SgfI-MluI
• OTI Disclaimer: Our molecular clone sequence data has been matched to the sequence identifier above as a point of reference. Note that the complete sequence of this clone is largely the same as the reference sequence but may contain minor differences , e.g., single nucleotide polymorphisms (SNPs).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as 10 ug dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials.

Reference Data

• RefSeq: NM_001024599.3
• Summary: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. This structure consists of approximately 146 bp of DNA wrapped around a nucleosome, an octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-dependent histone that is a member of the histone H2B family and is found in a histone cluster on chromosome 1. [provided by RefSeq, Aug 2015]
• Locus ID: 440689